The SAPK/JNK pathway initiates apoptosis upon exposure to radiation, UV exposure, heat shock, oxidative stress, and other stressors. Upon exposure to environmental stress, the SAPK/JNK signaling pathway sequentially activates the proteins MEKK1, SEK1, SAPK, and c-Jun. Upstream activators of the SAPK/JNK cascade include ceramide, small GTP-binding proteins such as Rac1 and Cdc42Hs, Ask1, and caspases. MKK7 is also a major and direct SAPK/JNK activator in the TNFα or environmental stress signaling pathways, where its kinase activity directly phosphorylates SAPK/JNK. This relationship between MKK7 and SAPK appears to be evolutionarily conserved, as it is preserved in their Drosophila homologues, Hep and DJNK, respectively.
Predicted to work with mouse, rat and other homologues.
Detection
Anti-Rabbit IgG
Clonality
Monoclonal
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human phospho SAPK/JNK
Formulation
1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype
Rabbit IgGk
Preparation
Protein A+G
Recommended Usage
1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage
-20ºC
Pseudonyms
Mitogen-activated protein kinase 8, MAPK8, Stress-activated protein kinase 1c, SAPK1c, c-Jun N-terminal kinase 1, JNK1, PRKM8
Verheij M, Bose R, Lin XH, et al. (1996) Nature. 380: 75-79. Verheij M, Ruiter GA, Zerp SF, et al. (1998) Radiotherapy and Oncology. 47: 225-232. Moriguchi T, Toyoshima F, Masuyama N, et al. (1997) The EMBO Journal 16: 7045-7053.