Quinolinic Acid ELISA Kit
Katalog-Nummer OKCD02284
Size : 96Wells
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Quinolinic Acid ELISA Kit (OKCD02284) |
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| COA Datasheet | Click Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Quinolinic Acid (QA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Quinolinic Acid (QA) and unlabeled Quinolinic Acid (QA) (Standards or samples) with the pre-coated antibody specific to Quinolinic Acid (QA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Quinolinic Acid (QA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Quinolinic Acid (QA) in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 1.23-100ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 89-00-9 PubChem: 1066 | ||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.58 ng/mL | ||||||||||||||||||||||
| Publications | Abdur Rahman, Muddanna S. Rao & Khalid M. Khan. Intraventricular infusion of quinolinic acid impairs spatial learning and memory in young rats: a novel mechanism of lead-induced neurotoxicity. J Neuroinflammation. 15, 263 (2018). PMC6137743 Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in a Triple Transgenic Mouse Model of Alzheimer Disease. Am J Pathol. 189, 1435-1450 (2019). 30980800 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Competitive Inhibition Immunoassay | ||||||||||||||||||||||
| Description |
| Gene Symbol | QA |
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| Alias Symbols | Quinolinic acid; 2,3-pyridinedicarboxylic acid; Pyridine-2,3-dicarboxylic acid; 89-00-9; Quinolinate; Pyridine-2,3-dicarboxylate |
| Protein Name | Quinolinic Acid |
| Description of Target | Quinolinic acid is a metabolite of tryptophan with a possible role in neurodegenerative disorders. Elevated CSF levels of quinolinic acid are correlated with the severity of neuropsychological deficits in patients who have AIDS. |