QuantiChrom™ Glyoxalase I Assay Kit

Katalog-Nummer DGLO-100

Size : 100tests

Marke : BioAssay Systems

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Telefonnummer : +1 850 650 7790

QuantiChrom™ Glyoxalase I Assay Kit



Application

  • For quantitative determination of glyoxalase enzyme activity.

Key Features

  • Fast and sensitive. Detection limit of 4 U/L GLO-1 activity.
  • Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD240nm (Use a UV plate, e.g. 96-well UV plate, cat# P96UV)

Samples

  • Enzyme preparations or biological samples

Species

  • All

Procedure

  • 30 min

Size

  • 100 tests

Detection Limit

  • 4 U/L

Shelf Life

  • 12 months

More Details

GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer. Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. BioAssay Systems Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S-lactoylglutathione, by measuring the change in absorbance at 240 nm.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

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Glyoxalase I Assay Kit
Catalog No: DGLO-100 Category : Enzyme Activity Tags DGLO-100, Enzyme Activity, Glyoxalase I assay kits, Glyoxalase I determination kits, Glyoxalase I measurement kits, Glyoxalase I test kits, QuantiChrom Glyoxalase I Assay Kits, QuantiChromTM Glyoxalase I Assay Kit

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EDLC-100
 100tests