ProxUp2 medium support the growth of Evercyte ́s human renal proximal tubular epithelial cells RPTEC/TERT1.
General information
Cat#: MHT-003-2 / MHT-003-2-B + MHT-003-S
Medium composition
DMEM/F12 (1:1) (PAN-Biotech, Cat# P04-41154)
10 mM HEPES-buffer (Sigma-Aldrich, Cat# H0887, ready-to-use)
0.5% FBS (PAN Biotech, Cat# P30-3031, ready-to-use, store at 4°C after thawing)
10 ng/ml hEGF (Sigma-Aldrich, Cat# E9644)
5 pM 3,3′,5-Triiodo-L-thyronine sodium salt (T3, Sigma-Aldrich, Cat# T6397)
3.5 µg/ml L-Ascorbic Acid (Sigma-Aldrich, Cat# A4544)
5 µg/ml Transferrin Holo (Merck Millipore, Cat# 616424)
25 ng/ml Prostaglandine E1 (Sigma-Aldrich, Cat# P8908)
25 ng/ml Hydrocortisone (Sigma-Aldrich, Cat# H0396)
8.65 ng/ml Sodium-Selenite (Sigma-Aldrich, Cat# S5261)
5 µg/ml Insulin (Sigma-Aldrich, Cat# I9278, ready-to-use)
100 µg/ml G418 (InvivoGen, Cat# ant-gn-5, ready-to-use)
FAQs
Preparation of ProxUp2 medium
The ProxUp2 medium for cultivation of RPTEC/TERT1 cells can either be ordered from Evercyte as ready-to-use medium (Cat# MHT-003-2) or as basal medium (MHT-003-2-B) + Supplements (MHT-003-S) or can be prepared by mixing the following components:
take one bottle (500 ml) of DMEM/F12 (1:1) (PAN-Biotech, Cat# P04-41154) |
add 2.5ml of FBS (ready-to-use, PAN Biotech, Cat# P30-3031)
Storage conditions
Store the basal medium at 4°C for up to 6 months and the supplements -20°C for up to 6 months.
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