Polymyxin B (PMB) Mouse Monoclonal Antibody [Clone ID: 45]

Specifications

Product Data
Clone Name	45
Applications	ELISA, WB
Recommended Dilution	Western blotting: Use 1/50 as starting working dilution. Immunoassays.
Reactivities	Broad
Host	Mouse
Isotype	IgM
Clonality	Monoclonal
Specificity	This Monoclonal antibody clone 45 reacts with Polymyxin B. It  binds to free Polymyxin B as well as to Polymyxin B already bound to LPS.  This antibody enables the possibilities to study quantitatively the interaction of PMB and LPS.
Formulation	PBS with 0.1% BSA and 0.02% Sodium Azide. State: Culture Medium with a Low Endotoxin.
Concentration	lot specific
Conjugation	Unconjugated
Storage	Store the antibody undiluted at 2-8°C.
Stability	Shelf life: one year from despatch.
Background	The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo.
Reference Data