Specifications

Product Data
C-Tag	His
E. coli Selection	Ampicillin (100 ug/mL)
Features	This vector is for E.coli expression. Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as tagged protein with a C-terminal His tag. Unlike other PrecisionShuttle vectors, this vector does not have an Sgf I site at the multiple cloning site. Instead a BseR I site can be used to generate Sgf I-compatible end for in-frame shuttling from TrueORF clones in pEntry vector. Detection and purification of the transgene are best done using an anti-His antibody or a nickel affinity column. If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal His-tagging vector, PS100030
Schematic of the multiple cloning sites