Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> Paclitaxel [33069-62-4]

Paclitaxel [33069-62-4]

Drucken Drucken
Melden Sie sich an

Katalog-Nummer HY-B0015-50mg

Size : 50mg

Marke : MedChemExpress

Contact local distributor :


Telefonnummer : +1 850 650 7790

Paclitaxel est un agent antinéoplasique naturel et stabilise la polymérisation de la tubuline. Paclitaxel peut provoquer à la fois l'arrêt mitotique et la mort cellulaire apoptotique. Paclitaxel induit également l'autophagie.

Paclitaxel ist ein natürlich vorkommendes antineoplastisches Mittel und stabilisiert die tubulinpolymerisation. Paclitaxel kann sowohl einen mitotischen Stillstand als auch apoptotic Zelltod verursachen. Paclitaxel induziert auch autophagy.

Paclitaxel is a naturally occurring antineoplastic agent and stabilizes tubulin polymerization. Paclitaxel can cause both mitotic arrest and apoptotic cell death. Paclitaxel also induces autophagy.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Paclitaxel Chemische Struktur

Paclitaxel Chemische Struktur

CAS. Nr. : 33069-62-4

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Paclitaxel Related Antibodies

Proliferation Assay
WB
Cell Viability Assay
    Paclitaxel (PTX; 3.125, 6.25, 12.5, 25, 50, 100 nM; 48 h) reduces the viability of Hela, SiHa, and C33A cells in a dose-dependent manner.(3.125 nM for Hela cells, 100 nM for SiHa and C33A cells)
    Paclitaxel (PTX; 50 nM; 48 h) increases the expression of Cleaved-caspase-9 and Cleaved-caspase-3 in Hela cells.
    Paclitaxel (25, 50 μM; 24 h) significantly increases the expression of caspase-3 in PK-15 cells.
    Paclitaxel (PTX; 0‑100 nM; 72 h) inhibits the viability of MDA‑MB‑231cells.
    Paclitaxel (CPT; 10-6 -12-10 M; 72 h) demonstrates a strong antitumor activity and an inhibitory of proliferation (IC50 = 6.28 nM) in U937 cells.
    Western blot image and statistical analysis of phosphorylated γH2AX levels in HT-29 cells with different treatments after 48 h. Myricetin and PTX used are 32 μM and 100 nM, respectively.
    The effects of CS1 on polymerized microtubules are also investigated using a cellular tubulin polymer assay. Nocodazole (1 μM) and Paclitaxel (1.5 μM) are used as depolymerization and polymerization controls, respectively.
    Synergistic inhibition of colony formation of ATC cells by E7080 and Paclitaxel. Representative images of colony formation in C643 cells treated with vehicle control (DMSO) or E7080 and Paclitaxel at the indicated concentrations, individually or in combination, are shown in left panel. Quantitative analysis of colony numbers is shown in right panel.

    • Biologische Aktivität

    • Reinheit & Dokumentation

    • Verweise

    • Kundenbewertung

    Paclitaxel is a naturally occurring antineoplastic agent and stabilizes tubulin polymerization. Paclitaxel can cause both mitotic arrest and apoptotic cell death. Paclitaxel also induces autophagy.

    Traditional Cytotoxic Agents

     

    In Vitro

    Paclitaxel (20 nM; 48 hours) induces programmed cell death and exists a block at the G2/M phase of the cell cycle.
    ? Paclitaxel (20 nM; 48 hours) induces a consistent increase in the level of p53.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Paclitaxel Related Antibodies

    Apoptosis Analysis

    Cell Line: MCF-7, MDA-MB-231 cells
    Concentration: 20 nM
    Incubation Time: 48 hours
    Result: Induced programmed cell death.

    Cell Cycle Analysis

    Cell Line: MCF-7, MDA-MB-231 cells
    Concentration: 20 nM
    Incubation Time: 48 hours
    Result: >60% of MCF-7 cells and 50% of MDA-MB-231 cells were in the G2/M phase following 24 h treament.

    Western Blot Analysis

    Cell Line: MCF-7 cells (harboring wild-type p53)
    Concentration: 20 nM
    Incubation Time: 48 hours
    Result: Induced a consistent increase in the level of p53.
    In Vivo

    Paclitaxel (1-20 mg/kg; i.p.; 1 time/2 days for five cycles) obviously induces liver metastases at the low-Paclitaxel group with little influence on primary tumor growth.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: MDA-231 xenograft-bearing mice
    Dosage: 1, 20 mg/kg
    Administration: Intraperitoneal injection; five cycles (1 time/2 days)
    Result: Liver metastases were obviously induced in the low-PTX (1 mg/kg) group with little influence on primary tumor growth compared with high-PTX group.

    853.91

    Solid

    C47H51NO14

    33069-62-4

    O=C(C1=CC=CC=C1)N[C@@H](C2=CC=CC=C2)[C@H](C(O[C@@H]3C(C)=C([C@@H](OC(C)=O)C([C@@]4(C)[C@]([C@@](CO5)(OC(C)=O)[C@@]5(03)C[C@@H]4O)(03)[C@@H]6OC(C7=CC=CC=C7)=O)=O)C(C)(C)[C@@]6(O)C3)=O)O
    • Terpenoids
    • Diterpenoids
    • Plants
    • Taxaceae
    • Taxus chinensis (Pilger) Rehd.
    • Plants
    • Illiciaceae
    • Taxus chinensis (Pilger) Rehd.

    Room temperature in continental US; may vary elsewhere.

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    In Vitro: 

    DMSO : 100 mg/mL (117.11 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.1711 mL 5.8554 mL 11.7108 mL
    5 mM 0.2342 mL 1.1711 mL 2.3422 mL
    10 mM 0.1171 mL 0.5855 mL 1.1711 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  50% PEG300    50% saline

      Solubility: 10 mg/mL (11.71 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  corn oil

      Solubility: 10 mg/mL (11.71 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (2.44 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.08 mg/mL (2.44 mM); Suspended solution; Need ultrasonic

    • 5.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (2.44 mM); Clear solution

    *All of the co-solvents are available by MCE.

    • [1]. Choi YH, et al. Paclitaxel-induced growth arrest and apoptosis is associated with the upregulation of the Cdk inhibitor, p21WAF1/CIP1, in human breast cancer cells. Oncol Rep. 2012 Dec;28(6):2163-9.  [Content Brief]

      [2]. Dziadyk JM, et al. Paclitaxel-induced apoptosis may occur without a prior G2/M-phase arrest. Anticancer Res. 2004 Jan-Feb;24(1):27-36.  [Content Brief]

      [3]. Li Q, et al. Low doses of paclitaxel enhance liver metastasis of breast cancer cells in the mouse model. FEBS J. 2016 Aug;283(15):2836-52.  [Content Brief]

      [4]. Pan Z, et al. Paclitaxel attenuates Bcl-2 resistance to apoptosis in breast cancer cells through an endoplasmic reticulum-mediated calciumrelease in a dosage dependent manner. Biochem Biophys Res Commun. 2013 Feb 13. pii: S0006-291X(13)00259-3.  [Content Brief]

      [5]. Cadamuro M, et al. Low dose paclitaxel reduces S100A4 nuclear import to inhibit invasion and hematogenous metastasis of cholangiocarcinoma. Cancer Res. 2016 Jun 21.  [Content Brief]

      [6]. Li Q, et al. Low doses of paclitaxel enhance liver metastasis of breast cancer cells in the mouse model. FEBS J. 2016 Jun 16.  [Content Brief]

      [7]. Yilmaz E, et al. Sensory neuron subpopulation-specific dysregulation of intracellular calcium in a rat model of chemotherapy-induced peripheral neuropathy. Neuroscience. 2015 Aug 6;300:210-8.  [Content Brief]

      [8]. Jing C, et al. E7080 enhances the antitumor effects of paclitaxel in anaplastic thyroid cancer. Am J Cancer Res. 2017 Apr 1;7(4):903-912.  [Content Brief]

    • [1]. Choi YH, et al. Paclitaxel-induced growth arrest and apoptosis is associated with the upregulation of the Cdk inhibitor, p21WAF1/CIP1, in human breast cancer cells. Oncol Rep. 2012 Dec;28(6):2163-9.

      [2]. Dziadyk JM, et al. Paclitaxel-induced apoptosis may occur without a prior G2/M-phase arrest. Anticancer Res. 2004 Jan-Feb;24(1):27-36.

      [3]. Li Q, et al. Low doses of paclitaxel enhance liver metastasis of breast cancer cells in the mouse model. FEBS J. 2016 Aug;283(15):2836-52.

      [4]. Pan Z, et al. Paclitaxel attenuates Bcl-2 resistance to apoptosis in breast cancer cells through an endoplasmic reticulum-mediated calciumrelease in a dosage dependent manner. Biochem Biophys Res Commun. 2013 Feb 13. pii: S0006-291X(13)00259-3.

      [5]. Cadamuro M, et al. Low dose paclitaxel reduces S100A4 nuclear import to inhibit invasion and hematogenous metastasis of cholangiocarcinoma. Cancer Res. 2016 Jun 21.

      [6]. Li Q, et al. Low doses of paclitaxel enhance liver metastasis of breast cancer cells in the mouse model. FEBS J. 2016 Jun 16.

      [7]. Yilmaz E, et al. Sensory neuron subpopulation-specific dysregulation of intracellular calcium in a rat model of chemotherapy-induced peripheral neuropathy. Neuroscience. 2015 Aug 6;300:210-8.

      [8]. Jing C, et al. E7080 enhances the antitumor effects of paclitaxel in anaplastic thyroid cancer. Am J Cancer Res. 2017 Apr 1;7(4):903-912.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    • Cytoskeleton Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related Apoptosis Autophagy
    • Microtubule/Tubulin ADC Cytotoxin Apoptosis Autophagy
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Sie könnten auch an folgenden Produkten interessiert sein:



    Katalog-Nummer
    Beschreibung
    Cond.
    Preis zzgl. MwSt.