Omalizumab (Xolair) Pharmacokinetic ELISA
Katalog-Nummer EL-1611-152
Size : 1X96wells
Marke : AffinityImmuno
Contact local distributor :
Telefonnummer : +1 850 650 7790
The Omalizumab PK ELISA kit is designed to measure Omalizumab in serum or plasma with high specificity and sensitivity through sandwich technique.
INTRODUCTION
The Omalizumab PK ELISA kit is designed to measure Omalizumab in serum or plasma with high specificity and sensitivity. This sandwich ELISA design employs a Omalizumab specific monoclonal antibody for capture and a specific monoclonal antibody for detection.
PRINCIPLE OF THE ASSAY
This assay employs the sandwich enzyme immunoassay technique. Anti- Omalizumab is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Omalizumab present in biological matrices is bound by the immobilized anti- Omalizumab antibody. After washing away any unbound substances, enzyme linked anti- Omalizumab antibody is added to the wells. This antibody is developed and purified specifically against Xolair® (domain residing in Fc portion of the Xolair® molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Omalizumab present in test samples. The color development is stopped and the intensity of the color is measured
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Catalog Number:
EL-1611-152 -
Application(s)
measure free Omalizumab -
Target Protein
xolair -
Alternative Name of Target
omalizumab -
Size
96 wells -
Sensitivity
detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
1.5ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Product Specifications
-
Catalog Number:
EL-1611-152 -
Application(s)
measure free Omalizumab -
Target Protein
xolair -
Alternative Name of Target
omalizumab -
Size
96 wells -
Sensitivity
detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
1.5ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3