MYPT1, phosphorylated (Thr853/Thr850) (Myosin Phosphatase 1)

Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, as previously shown. To understand the role of MYPT1 in the regulation of the cytoskeleton in human diseases, a 4855-bp cDNA was cloned for human MYPT1 using the rat MYPT1 cDNA as probe. Sequencing analysis has revealed that human MYPT1 contains 1030 amino acid residues with a calculated molecular mass of ~115kD. Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybrid mapping has shown that the human MYPT1 gene is located very near the highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106.

Suitable for use in Western Blot. Other applications have not been tested.

Western Blot: 0.5-2ug/ml. Detects MYPT1 by preincubation with ROKa/ROK-II active or C2C12 lysates treated with pervanadate.
Optimal dilutions to be determined by the researcher.

I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Pab

IgG

rabbit

~1mg/ml

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

Purified by Protein A affinity chromatography.

Synthetic peptide corresponding to aa845-855, C-EKRRS[pT]GVSFW of chicken MYPT1 with an N-terminal cysteine added for conjugation purposes. Phosphorylation site corresponds to Thr853 in human MYPT1.

Recognizes recombinant truncated MYPT1 phosphorylated on Thr853, MW ~80kD. Species Crossreactivity

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.