MPTP (hydrochloride) [23007-85-4]
Katalog-Nummer HY-15608-100mg
Size : 100mg
Marke : MedChemExpress
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Chlorhydrate de MPTP est une neurotoxine de dopamine pénétrante dans le cerveau, induisant la maladie de Parkinson. Chlorhydrate de MPTP, précurseur du MPP+, induit l'apoptose.
MPTP-Hydrochlorid ist ein hirneindringendes dopamine-Neurotoxin, das die Parkinson-Krankheit auslöst. MPTP-Hydrochlorid, ein Vorläufer von MPP+, induziert apoptosis.
MPTP hydrochloride is a brain penetrant dopamine neurotoxin. MPTP hydrochloride can be used to induces Parkinson’s Disease model. MPTP hydrochloride, a precusor of MPP+, induces apoptosis. MPTP hydrochloride has been verified by MCE with professional biological experiments.
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MPTP hydrochloride Chemische Struktur
CAS. Nr. : 23007-85-4
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- Immunofluorescence for TH. The intranigral Apelin-13 injection significantly inhibits MPTP-induced the neurodegeneration of dopaminergic neurons in the SNpc.
- Effect of treatment with Rapamycin, trehalose, or their combination on tyrosine hydroxylase (TH) expression in the striatum in MPTP-induced mouse model of Parkinson’s disease.
- Effect of treatment with Rapamycin, trehalose, or their combination on autophagy activity measured by quantified immunoreactivity of LC3-II in the s. nigra. MPTP is administered at the dose of 20 mg/kg (i.p., daily) for 4 days to induce PD-like pathology.
- RNA 5hmC decreases in a MPTP-induced Parkinson's disease mouse model. MPTP (i.p. 60 mg/kg) is injected to induce Parkinson's disease model in mice. At 24 h after last MPTP injection, open field test is performed. After behavioral tests, the hippocampus (Hipo), the substantia nigra (SN), the striatum (Str), and the cortex (Ctx) are collected and total RNA is extracted. Total 100 ng RNA is used for dot blot analysis to detect 5hmC abundance in RNA samples from different brain regions. Methylene bl
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| MPTP hydrochloride is a brain penetrant dopamine neurotoxin. MPTP hydrochloride can be used to induces Parkinson’s Disease model. MPTP hydrochloride, a precusor of MPP+, induces apoptosis. MPTP hydrochloride has been verified by MCE with professional biological experiments. | |
| In Vitro | Pretreatment with 50 mM 4-phenylpyridine, reduces IC50 (concentration for 50% inhibition of twitch amplitude) values of MPTP from 53 to 18 mM and d-tubocurarine from 0.7 to 0.3 mM, respectively, in mouse phrenic nerve-diaphragm. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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| For the preparation of the LPS rat model and the MPTP mouse model, the treatments of the animals are performed. Briefly, adult rats receive unilateral injections of LPS (0.5 μL of 10 μg/μL diluted in 0.9% saline) into the medial forebrain bundle (MFB) at the following coordinates, AP-4.2 mm, L 1.5 mm, and V 7.8 mm, and into the contralateral side with the same volume of 0.9% saline. Adult mice are administered intraperitoneal injections of MPTP of 25 mg/kg per day for five continuous days, and the same volume of saline is injected as a control. All the animals are sacrificed at week 1, 2, 3, or 4 after the LPS or MPTP injections. The brain samples are collected for the subsequent immunohistochemistry and western blot experiments. MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz. |
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[1]. Langston J W, Irwin I. MPTP Neurotoxicity: An Overview and Characterization of Phases of Toxicity. II. Selective Accumulation of MPP in the Substantia Nigra: A Key to Neurotoxicity (Question). Life Sci., 1985, 36, No. 3, 201-12.
[2]. Hsu K S, et al. Potentiation of MPTP by 4-Phenylpyridine on the Neuromuscular Blockade in Mouse Phrenic Nerve-Diaphragm. Neuropharmacology, 1993, 32, No. 9, 877-83.
[3]. Sun XL, et al. Gas1 up-regulation is inducible and contributes to cell apoptosis in reactive astrocytes in the substantia nigra of LPS and MPTP models. J Neuroinflammation. 2016 Jul 8;13(1):180.
[4]. Jackson-Lewis V, Przedborski S. Protocol for the MPTP mouse model of Parkinson's disease. Nat Protoc. 2007;2(1):141-51.
[5]. Rabaneda-Lombarte N, et al. The CD200R1 microglial inhibitory receptor as a therapeutic target in the MPTP model of Parkinson's disease. J Neuroinflammation. 2021 Apr 6;18(1):88.
[6]. Lee, et al. MPTP-driven NLRP3 inflammasome activation in microglia plays a central role in dopaminergic neurodegeneration. Cell Death Differ. 2019 Jan;26(2):213-228.
[7]. Zhang QS, et al. Reassessment of subacute MPTP-treated mice as animal model of Parkinson's disease. Acta Pharmacol Sin. 2017 Oct;38(10):1317-1328.
[8]. Hammock BD, et al., A sheep model for MPTP induced Parkinson-like symptoms. Life Sci. 1989;45(17):1601-8.
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|
| DMSO / H2O | 1 mM | 4.7683 mL | 23.8413 mL | 47.6826 mL | 119.2066 mL |
| 5 mM | 0.9537 mL | 4.7683 mL | 9.5365 mL | 23.8413 mL | |
| 10 mM | 0.4768 mL | 2.3841 mL | 4.7683 mL | 11.9207 mL | |
| 15 mM | 0.3179 mL | 1.5894 mL | 3.1788 mL | 7.9471 mL | |
| 20 mM | 0.2384 mL | 1.1921 mL | 2.3841 mL | 5.9603 mL | |
| 25 mM | 0.1907 mL | 0.9537 mL | 1.9073 mL | 4.7683 mL | |
| 30 mM | 0.1589 mL | 0.7947 mL | 1.5894 mL | 3.9736 mL | |
| 40 mM | 0.1192 mL | 0.5960 mL | 1.1921 mL | 2.9802 mL | |
| 50 mM | 0.0954 mL | 0.4768 mL | 0.9537 mL | 2.3841 mL | |
| H2O | 60 mM | 0.0795 mL | 0.3974 mL | 0.7947 mL | 1.9868 mL |
| 80 mM | 0.0596 mL | 0.2980 mL | 0.5960 mL | 1.4901 mL | |
| 100 mM | 0.0477 mL | 0.2384 mL | 0.4768 mL | 1.1921 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
- Induced Disease Models Products
- Nervous System Disease Models
- Parkinson's Disease Models
- GPCR/G Protein Neuronal Signaling Apoptosis
- Dopamine Receptor Apoptosis
- Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
MPTP23007-85-4Dopamine ReceptorApoptosisbrainpenetrantneurotoxinParkinson’sDiseaseInhibitorinhibitorinhibit
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- Produktname:
- MPTP hydrochloride
- Art. -Nr.:
- HY-15608
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