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> Mouse Monoclonal Anti-AAV VP1/VP2/VP3, supernatant [Clone B1]

Mouse Monoclonal Anti-AAV VP1/VP2/VP3, supernatant [Clone B1]

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Katalog-Nummer 65158

Size : 5mL

Marke : Progen

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anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, supernatant

Datasheet
Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with denatured VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application Affinity chromatography, Dot blot, ICC/IF, IP, WB
Purification Hybridoma cell culture supernatant
Reactivity AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVDJ, AAVrh10
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone B1
Immunogen AAV2 capsids
Formulation Contains 0.09% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Affinity Chromatography Assay dependent
Dot Blot Assay dependent (denaturing conditions)
Immunoprecipitation (IP) Assay dependent (precipitation of mainly free VP proteins)
Western Blot (WB) 1:25-1:200
Background

The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

References/Publications (60)
Publication
Species
Application
Publication Coughlin G.M., et al., Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo., Nat Biotechnol, (2025).
Species AAV9
Application IF
Publication Zhou, Y. et al. Adeno-associated virus serotype 2 capsids with proteolytic cuts by trypsin remain intact and potent. Gene Ther 1–11 (2024) doi:10.1038/s41434-024-00507-4.
Species AAV2
Publication Yamaguchi, Y., et al., Glycosylation of recombinant adeno-associated virus serotype 6., Mol Ther Methods Clin Dev 32, 101256, (2024).
Species AAV6
Application WB
Publication Liu, H., et al., Producing high-quantity and high-quality recombinant adeno-associated virus by low-cis triple transfection., Mol Ther Methods Clin Dev 32, 101230, (2024).
Species AAV9
Application WB
Publication Guapo, F., et al., A Direct Comparison of rAAV5 Variants Derived from the Baculovirus Expression System Using LC-MS Workflows Demonstrates Key Differences in Overall Production Yield, Product Quality and Vector Efficiency., Int J Mol Sci 25, (2024).
Species AAV5
Application WB
Publication Leray, A., et al., Novel chemical tyrosine functionalization of adeno-associated virus improves gene transfer efficiency in liver and retina., Biomed Pharmacother 171, 116148, (2024).
Species AAV2
Application WB
Publication Chang, H. et al. Non-canonical amino acid incorporation into AAV5 capsid enhances lung transduction in mice. Mol Ther Methods Clin Dev 31, (2023).
Species AAV5
Application WB
Publication Theuerkauf, S.A. et al., AAV vectors displaying bispecific DARPins enable dual-control targeted gene delivery., Biomaterials 303, 122399, (2023).
Species AAV2
Application WB
Publication Eriksson, R. A. E. et al. Optimized riboswitch-regulated AAV vector for VEGF-B gene therapy. Front Med (Lausanne) 9, (2022).
Species AAV6
Application WB
Publication Su, W. et al. Self-attenuating adenovirus enables production of recombinant adeno-associated virus for high manufacturing yield without contamination. Nat Commun 13, (2022).
Species AAV2
Application WB
Publication Hörner, M. & Weber, W. Spatially Defined Gene Delivery into Native Cells with the Red Light-Controlled OptoAAV Technology. Curr Protoc 2, (2022).
Species AAV2
Application WB
Publication Emmanuel, S. N. et al. Structurally Mapping Antigenic Epitopes of Adeno-associated Virus 9: Development of Antibody Escape Variants. J. Virol. 96, (2022).
Species AAV5, 9
Application dot blot
Publication Jacob, A. et al. Homologous Recombination Offers Advantages over Transposition-Based Systems to Generate Recombinant Baculovirus for Adeno-Associated Viral Vector Production. Biotechnol J 16, (2021).
Species AAV2
Application WB
Publication Elmore, Z. C. et al. The membrane associated accessory protein is an adeno-associated viral egress factor. Nat Commun 12, (2021).
Species AAV8
Application WB
Publication Guy, J., et al., Amelioration of Leigh syndrome induced by mouse blastocyst complementation with a mutant human mitochondrial ATP synthase 6, Research Square, (2021).
Species AAV9
Application WB, EM
Publication Kuklik, J. et al. Development of a bispecific antibody-based platform for retargeting of capsid modified aav vectors. Int. J. Mol. Sci. 22, 8355 (2021).
Species AAV2
Application WB
Publication Galibert, L. et al. Functional roles of the membrane-associated AAV protein MAAP. Sci. Rep. 11, (2021).
Species AAV2
Application WB
Publication Meng, Y. et al. Cell-penetrating peptides enhance the transduction of adeno-associated virus serotype 9 in the central nervous system. Mol Ther Methods Clin Dev. 21, 28-41(2021).
Species AAV9
Application IHC/IF
Publication Feiner, R. C., Kemker, I., et al. EGFR-Binding Peptides: From Computational Design towards Tumor-Targeting of Adeno-Associated Virus Capsids. Int J Mol Sci. 2020-12-15.
Species AAV2
Application WB
Publication Lins-Austin, B. et al. Adeno-Associated Virus (AAV) Capsid Stability and Liposome Remodeling During Endo/Lysosomal pH Trafficking. Viruses, 12, 668 (2020).
Species AAV1, 2, 5, 8
Application dot blot
Publication Davidsson, M. et al. A comparison of AAV-vector production methods for gene therapy and preclinical assessment. Sci Rep 10, (2020).
Species AAV2/9
Application WB
Publication Feiner, R. C. et al. Egfr-binding peptides: From computational design towards tumor-targeting of adeno-associated virus capsids. Int J Mol Sci 21, 1–22 (2020).
Species AAV2
Application WB
Publication Zhang, R. et al. Divergent engagements between adeno-associated viruses with their cellular receptor AAVR. Nat.Commun. 10, 3760 (2019)
Species AAV
Application WB
Publication Le, D. et al. Adeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly. Sci.Rep. 9, 18631 (2019)
Species E. coli
Application WB
Publication Blessing, D. et al. Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells. Mol.Ther.Methods.Clin.Dev. 13, 14-26 (2019)
Species human
Application WB
Publication Jose, A. et al. High-Resolution Structural Characterization of a New Adeno-associated Virus Serotype 5 Antibody Epitope toward Engineering Antibody-Resistant Recombinant Gene Delivery Vectors. J. Virol. 93, 1394–1412 (2019).
Species AAV1, 2, 3, 5, 6, 7, 8, 9, rh10
Application dot blot
Publication Wu, Y. et al. Development of Versatile and Flexible Sf9 Packaging Cell Line-Dependent OneBac System for Large-Scale Recombinant Adeno-Associated Virus Production. Hum.Gene.Ther.Methods. 30, 172-183 (2019)
Species human
Application WB
Publication Feiner, R. et al. rAAV Engineering for Capsid-Protein Enzyme Insertions and Mosaicism Reveals Resilience to Mutational, Structural and Thermal Perturbations. Int.J.Mol.Sci. 20, (2019)
Species human
Application WB
Publication Tomono, T. et al. Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9. Mol.Ther.Methods.Clin.Dev. 11, 180-190 (2018)
Species AAV9
Application WB
Publication Maddalena, A. et al. High-Throughput Screening Identifies Kinase Inhibitors That Increase Dual Adeno-Associated Viral Vector Transduction In Vitro and in Mouse Retina. Hum.Gene.Ther. 29, 886-901 (2018).
Application IP
Publication Galibert, L. et al. Origins of truncated supplementary capsid proteins in rAAV8 vectors produced with the baculovirus system. PLoS.One. 13, e0207414 (2018).
Application WB
Publication Schiller, L. et al. Enhanced Production of Exosome-Associated AAV by Overexpression of the Tetraspanin CD9. Mol.Ther.Methods.Clin.Dev. 9, 278-287 (2018).
Species AAV2
Application WB
Publication François, A. et al. Accurate Titration of Infectious AAV Particles Requires Measurement of Biologically Active Vector Genomes and Suitable Controls. Mol. Ther. - Methods Clin. Dev. 10, 223–236 (2018).
Species AAV8
Application WB
Publication Savy, A. et al. Genetics instability of wtAAV2 genome and AAV promoter activities in the Baculovirus/Sf9 cells system. PLoS.One. 13, e0199866 (2018)
Species AAV2
Application WB
Publication Wu, Y. et al. A Recombinant Baculovirus Efficiently Generates Recombinant Adeno-Associated Virus Vectors in Cultured Insect Cells and Larvae. Mol.Ther.Methods.Clin.Dev. 10, 38-47 (2018).
Species AAV2
Application WB
Publication Earley, L. F. et al. Adeno-associated Virus (AAV) Assembly-Activating Protein Is Not an Essential Requirement for Capsid Assembly of AAV Serotypes 4, 5, and 11. J. Virol. 91, 1980–1996 (2017).
Species AAV5
Application WB
Publication Satkunanathan, S., Thorpe, R. & Zhao, Y. The function of DNA binding protein nucleophosmin in AAV replication. Virology 510, 46–54 (2017).
Species AAV2
Application WB
Publication Savy, A. et al. Impact of Inverted Terminal Repeat Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum.Gene.Ther.Methods. 28, 277-289 (2017).
Species AAV8
Application WB
Publication Meliani, A. et al. Enhanced liver gene transfer and evasion of preexisting humoral immunity with exosome-enveloped AAV vectors. Blood.Adv. 1, 2019-2031 (2017).
Application WB
Publication Tseng, Y.-S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Methods 236, 105-110 (2016).
Species AAV1,AAV2,AAV3,AAV5,AAV6,AAV7,AAV8,AAV9
Application dot blot
Publication Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
Species AAV1,AAV2,AAV3,AAV5,AAV6,AAV7,AAV8,AAV9,AAVrh10
Application WB
Publication Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1–12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212–222 (2014).
Species AAV1,AAV2,AAV3,AAV5,AAV6,AAV7,AAV8,AAV9,AAVrh10
Application WB
Publication Jin, L.-F. et al. Ultrasound Targeted Microbubble Destruction Stimulates Cellular Endocytosis in Facilitation of Adeno-Associated Virus Delivery. Int. J. Mol. Sci 14, 9737–9750 (2013).
Species AAV5
Application WB
Publication Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038–13048 (2012).
Species AAV2
Application WB,dot blot
Publication Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686–12697 (2011).
Species AAV1,AAV2,AAV5,AAV8,AAV9
Application WB
Publication Stieger, K. et al. Detection of intact rAAV particles up to 6 years after successful gene transfer in the retina of dogs and primates. Mol. Ther. 17, 516–523 (2009).
Species AAV5
Application WB
Publication Chen, H. Intron splicing-mediated expression of AAV rep and cap genes and production of AAV vectors in insect cells. Mol. Ther. 16, 924–930 (2008). sw-product.general.copy
Species AAV2
Application WB
Publication Fragkos, M., Breuleux, M., Clément, N. & Beard, P. Recombinant Adeno-Associated Viral Vectors Are Deficient in Provoking a DNA Damage Response. J. Virol. 82, 7379–7387 (2008).
Species AAV2
Application WB
Publication Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17–24 (2007).
Species AAV1,AAV5,AAV6
Application WB,ICC-IF
Publication Lochrie, M. A. et al. Mutations on the External Surfaces of Adeno-AssociatedVirus Type 2 Capsids That Affect Transduction andNeutralization. J. Virol. 80, 821–834 (2006).
Species AAV2
Application WB
Publication Grimm, D., Kay, M. A. & Kleinschmidt, J. A. Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6. Mol. Ther. 7, 839–850 (2003).
Species AAV2
Application WB
Publication Yan, Z. et al. Ubiquitination of both Adeno-Associated Virus Type 2 and 5 Capsid Proteins Affects the Transduction Efficiency of Recombinant Vectors. J. Virol. 76, 2043–2053 (2002).
Species AAV2,AAV5
Application WB
Publication Zhang, H.-G. et al. Addition of Six-His-Tagged Peptide to the C Terminus of Adeno-Associated Virus VP3 Does Not Affect Viral Tropism or Production. J. Virol. 76, 12023–12031 (2002).
Species AAV2
Application WB
Publication Rabinowitz, J. E. et al. Cross-Packaging of a Single Adeno-Associated Virus (AAV) Type 2 Vector Genome into Multiple AAV Serotypes Enables Transduction with Broad Specificity. J. Virol. 76, 791–801 (2002).
Species AAV2
Application WB
Publication Liu, X., Voulgaropoulou, F., Chen, R., Johnson, P. R. & Clark, K. R. Selective Rep-Cap gene amplification as a mechanism for high-titer recombinant AAV production from stable cell lines. Mol. Ther. 2, 394–403 (2000).
Species AAV2
Application WB,ICC-IF
Publication Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (20
Species AAV2
Application epitope mapping
Publication Ogasawara, Y. et al. Efficient Production of Adeno-associated Virus Vectors Using Split-type Helper Plasmids. Jpn. J. Cancer Res 90, 476–483 (1999).
Species AAV
Application WB
Publication Wistuba, A. et al. Subcellular Compartmentalization of Adeno-Associated Virus Type 2 Assembly. J. Virol. 71, 1341–1352 (1997).
Species AAV2
Application WB,IP,ICC-IF
Publication Wistuba, A., Weger, S., Kern, A., Rgen, J. & Kleinschmidt, A. Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins. J. Virol. 69, 5311–5319 (1995).
Species AAV2
Application WB
Publication Hölscher, C., Kleinschmidt, J. A. & Bürkle, A. High-level expression of adeno-associated virus (AAV) Rep78 or Rep68 protein is sufficient for infectious-particle formation by a rep-negative AAV mutant. J. Virol. 69, 6880–6885 (1995).
Species AAV2
Application ICC
Downloads
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File WB protocol
Category Protocol
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Datasheet
MSDS
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 

Anti-Adeno-Associated Virus Capsid Proteins Antibodies

For more information on the antibody binding epitopes of the capsid protein antibodies A1, B1 and A69 see the table below summarizing the published data from the following publication on the specific antibody binding sites:

Table Binding Sites of the AAV capsid protein antibodies

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Anti-Adeno-Associated Virus Particle Antibodies

For more information on the antibody binding epitopes on assembled AAV1, 2, 5, 8 and 9 capsids, see the tables below summarizing the published data from the following publications on the specific antibody binding sites:

summary binding epitopes


EM structures of assembled AAV1, 2, 4, 5, 6, and 8 capsids with Fab-fragments are available in the following publications:


PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Learn more

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AAV Antibodies


AAV Standards


AAV Dip'n'Check


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Dip’n’Check AAV1 and AAV6 - lateral flow assay
Dip’n’Check AAV1 and AAV6 - lateral flow assay
Cat. No. PR5216
  • Rapid lateral flow test for the detection of AAV1 and AAV6 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV1 and AAV6 capsids

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Dip’n’Check AAV2 and AAV3 - lateral flow assay
Dip’n’Check AAV2 and AAV3 - lateral flow assay
Cat. No. PR5223
  • Rapid lateral flow test for the detection of AAV2 and AAV3 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV2 and AAV3 capsids

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Dip’n’Check AAV5 - lateral flow assay
Dip’n’Check AAV5 - lateral flow assay
Cat. No. PR5205
  • Rapid lateral flow test for the detection of AAV5 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV5 capsids

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Dip’n’Check AAV8 - lateral flow assay
Dip’n’Check AAV8 - lateral flow assay
Cat. No. PR5208
  • Rapid lateral flow test for the detection of AAV8 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV8 capsids

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Dip’n’Check AAV9 - lateral flow assay
Dip’n’Check AAV9 - lateral flow assay
Cat. No. PR5209
  • Rapid lateral flow test for the detection of AAV9 capsids
  • Semi-quantitative AAV titer determination in cell lysates and purified preparations
  • Detection of full and empty AAV9 capsids

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OptiPrep™ - Iodixanol
OptiPrep™ - Iodixanol
Cat. No. 1893
  • Highly effective density gradient media for the separation of full and empty AAV particles
  • Designed for the purification of viruses (such as AAV)
  • Useful to isolate different cell types and organelles
  • Ready-made, sterile and endotoxin-tested solution of iodixanol

 

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