Mouse Kitlg (Kit ligand) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Kitlg
Gene ID:	17311
Uniprot ID:	SCF_MOUSE
Specificity:	This kit recognizes Mouse SCF in samples. No significant cross-reactivity or interference between Mouse SCF and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
Function	Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Protein Name	Kit Ligand Hematopoietic Growth Factor Kl Mast Cell Growth Factor Mgf Steel Factor Stem Cell Factor Scf C-Kit Ligand Cleaved Into - Soluble Kit Ligand Skitlg
Database Links	Reactome: R-MMU-1257604 Reactome: -MMU-1433557 Reactome: -MMU-1433559 Reactome: -MMU-5673001 Reactome: -MMU-6811558
Cellular Localisation	Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Isoform 2: Cytoplasm Cytoplasm Cytoskeleton Cell Membrane Cell Projection Lamellipodium Filopodium Soluble Kit Ligand: Secreted
Alternative ELISA Names	Kit Ligand ELISA kit Hematopoietic Growth Factor Kl ELISA kit Mast Cell Growth Factor ELISA kit Mgf ELISA kit Steel Factor ELISA kit Stem Cell Factor ELISA kit Scf ELISA kit C-Kit Ligand Cleaved Into - Soluble Kit Ligand ELISA kit Skitlg ELISA kit Kitlg ELISA kit Kitl ELISA kit Mgf ELISA kit Sl ELISA kit Slf ELISA kit
Specificity	This kit recognizes Mouse SCF in samples. No significant cross-reactivity or interference between Mouse SCF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-107	99
EDTA plasma (n=8)	93-108	99
Cell culture media (n=8)	96-111	101

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	54.42	99.24	334.47	51.50	102.41	345.92
Standard deviation	2.99	4.00	17.46	3.18	4.79	15.81
CV (%)	5.49	4.03	5.22	6.17	4.68	4.57

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance