Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended dilutions ELISA: 1:350,000. Western blot: 1:5,000 - 1:50,000. Immunohistochemistry: 1:1,000 - 1:5,000.
Reactivity
Mouse
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Mouse κ (kappa) light chain fragment
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Buffer
0.1 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, containing 0.01% (w/v) Sodium Azide as preservative State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction.
Presentation State
Aff - Purified
Concentration
lot specific
Purification
Immunoaffinity chromatography.
Conjugation
Unconjugated
Storage
Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.