Mouse Il3 (Interleukin-3) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Il3
Gene ID:	16187
Uniprot ID:	IL3_MOUSE
Specificity:	This kit recognizes Mouse IL-3 in samples. No significant cross-reactivity or interference between Mouse IL-3 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK.
Protein Name	Interleukin-3 Il-3 Hematopoietic Growth Factor Mast Cell Growth Factor Mcgf Multipotential Colony-Stimulating Factor P-Cell-Stimulating Factor
Cellular Localisation	Secreted
Alternative ELISA Names	Interleukin-3 ELISA kit Il-3 ELISA kit Hematopoietic Growth Factor ELISA kit Mast Cell Growth Factor ELISA kit Mcgf ELISA kit Multipotential Colony-Stimulating Factor ELISA kit P-Cell-Stimulating Factor ELISA kit Il3 ELISA kit Csfmu ELISA kit Il-3 ELISA kit
Specificity	This kit recognizes Mouse IL-3 in samples. No significant cross-reactivity or interference between Mouse IL-3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-103	96
EDTA plasma (n=8)	93-107	100
Cell culture media (n=8)	87-98	93

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	54.40	142.23	353.93	53.65	137.47	331.67
Standard deviation	2.85	7.14	18.40	2.80	5.99	10.02
CV (%)	5.24	5.02	5.20	5.22	4.36	3.02

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance