Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommended Dilution	Anti-Mouse IgG1 Biotin Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000.  Immunohistochemistry: 1/1,000-1/5,000.
Reactivities	Mouse
Host	Goat
Isotype	IgG
Clonality	Polyclonal
Immunogen	Native Mouse IgG1 heavy chain Protein.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: Biotin State: Aff - Purified State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (Immunoglobulin and Protease free) Preservative: 0.01% (w/v) Sodium Azide
Form	Aff - Purified
Reconstitution Method	Restore with 1.0 ml deionized water (or equivalent)
Concentration	lot specific
Purification	Immunoaffinity Chromatography using Mouse IgG1 coupled to Agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation	Biotin
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.