Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF
Recommended Dilution	Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilution(s): FLISA : 1:10,000 - 1:50,000 IF Microscopy : 1:1,000 - 1:5,000 Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
Reactivities	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Mouse IgG whole molecule.
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: TRITC State: Lyophilzed purified IgG fraction Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 3.0 moles TRITC per mole of Rabbit IgG
Reconstitution Method	Reconstitute with 1.0 ml of deionized water or equivalent.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	TRITC
Storage	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use.