Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

Specifications

Product Data
Recommended Dilution	Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Mouse IgG whole molecule
Specificity	This antibody detects Mouse IgG (H&L) F(ab')2 Fragment (ads. to Bovine, Equus, Human, Rabbit, Rat, Sheep). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Sodium Azide Label: TRITC State: Lyophilized Ig fraction Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.9 moles TRITC per mole of Goat IgG F(ab')2
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation	TRITC
Storage	Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.