Mouse IgG (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody

Specifications

Product Data
Recommended Dilution	Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Mouse IgG whole molecule.
Specificity	This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer. Label: TRITC State: Lyophilized F(ab')2 fragments. Label: Tetramethylrhodamine isothiocyanate (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 3.6 moles TRITC per mole of Goat IgG F(ab')2.
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	TRITC
Storage	Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.