Amount Used: 0.1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After washing the cells were then stained with goat anti-mouse Ig-R-PE, following which lymphocytes were gated and analyzed by flow cytometry.
Description: Pooled antisera from goats hyperimmunized with mouse IgG, IgM, and IgA; purified by affinity chromatography on mouse IgG, IgM, and IgA covalently linked to agarose; reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA; cross-adsorbed against human immunoglobulins and pooled sera for minimal reactivity
Reconstitution and Storage
Store at 2-8C
Immunogen
Mouse IgG, IgM, and IgA
Purification
Affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked toagarose.
Concentration
1.0 mg/mL
Specificity
Ig
Characterization
To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry toconform to characteristics of a standard reference reagent. Representative data are included in thisproduct insert
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Indirect immunostaining in conjunction with mouse monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting
Other Applications Data
Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage
- The purified (UNLB) antibody is supplied as 2.0 mg purified immunoglobulin in 2.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50%glycerol/50% PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The Beta- galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS/NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on thelabel if stored as directed.
Cross Absorption
Pooled human sera and purified human paraproteins.
Gene Full Name
B-cell antigen receptor complex-associated protein alpha chain
Protein interactions
Name
# of Products
AMBP
80
BLK
33
BLNK
60
CD19
68
CD5
81
CD79A
128
CD79B
88
FANCA
156
FCAR
36
FYN
597
GDF10
13
HCLS1
57
IGHM
57
IGJ
40
LCK
537
LYN
366
PIGR
21
PNMA1
34
PTPN6
335
PTPRC
283
SYK
252
TGM4
13
UBC
7030
Biological pathways
Name
# of Products
B cell differentiation
158
B cell proliferation
243
B cell receptor signaling pathway
217
Biological process
Name
# of Products
B cell activation
26
B cell differentiation
46
B cell proliferation
22
B cell receptor signaling pathway
35
Cell surface receptor signaling pathway
179
Cellular components
Name
# of Products
B cell receptor complex
6
External side of plasma membrane
261
Integral to membrane
2793
Membrane raft
140
Multivesicular body
18
Plasma membrane
2678
Protein function
Name
# of Products
Transmembrane signaling receptor activity
687
What is the species homology for "Goat Anti-Mouse Ig Antibody - FITC Conjugated (OASB01441)"?
The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "".
How long will it take to receive "Goat Anti-Mouse Ig Antibody - FITC Conjugated (OASB01441)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
What buffer format is "Goat Anti-Mouse Ig Antibody - FITC Conjugated (OASB01441)" provided in?
This item is provided in "". Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Goat Anti-Mouse Ig Antibody - FITC Conjugated (OASB01441)"?
This target may also be called "" in publications.
What is the shipping cost for "Goat Anti-Mouse Ig Antibody - FITC Conjugated (OASB01441)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
What is the guarantee for "Goat Anti-Mouse Ig Antibody - FITC Conjugated (OASB01441)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Goat Anti-Mouse Ig Antibody - FITC Conjugated (OASB01441)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "". Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Goat Anti-Mouse Ig Antibody - FITC Conjugated (OASB01441)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with ""?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with ""?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with ""?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with ""?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
Goat Anti-Mouse IgA Antibody - FITC Conjugated (OASB01536)
Catalog #: OASB01536
Conjugation: FITC: Fluorescein Isothiocyanate
Species Tested: Mouse
Application: FLISA, FC, IHC-F, IHC-P, ICC, WB
Size: 1mg
Goat Anti-Human IgA Antibody - PE Conjugated (OASB01840)
Catalog #: OASB01840
Conjugation: R-PE
Species Tested: Human
Application: FLISA, FC, MP
Format: The R-phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent.
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