Mouse Fn1 (Fibronectin) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Fn1
Gene ID:	14268
Uniprot ID:	FINC_MOUSE
Specificity:	This kit recognizes Mouse FN in samples. No significant cross-reactivity or interference between Mouse FN and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Sulfated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia.
Function	Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the Lilrb4a receptor, inhibiting Fcgr1/CD64-mediated monocyte activation. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Protein Name	Fibronectin Fn Cleaved Into - Anastellin
Database Links	Reactome: R-MMU-114608 Reactome: -MMU-1474228 Reactome: -MMU-1474244 Reactome: -MMU-1566977 Reactome: -MMU-202733 Reactome: -MMU-2129379 Reactome: -MMU-216083 Reactome: -MMU-3000170 Reactome: -MMU-3000178 Reactome: -MMU-354192 Reactome: -MMU-354194 Reactome: -MMU-372708 Reactome: -MMU-381426 Reactome: -MMU-5674135 Reactome: -MMU-8874081 Reactome: -MMU-8957275 Reactome: -MMU-9634597
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative ELISA Names	Fibronectin ELISA kit Fn Cleaved Into - Anastellin ELISA kit Fn1 ELISA kit
Specificity	This kit recognizes Mouse FN in samples. No significant cross-reactivity or interference between Mouse FN and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-102	93
EDTA plasma (n=8)	88-99	93
Cell culture media (n=8)	91-103	97

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	4.82	12.64	41.91	4.42	11.94	37.91
Standard deviation	0.30	0.56	1.88	0.28	0.60	1.65
CV (%)	6.22	4.43	4.49	6.33	5.03	4.35

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance