Mouse Anti-Human MUC1 Polyclonal Antibody

Figure 1 Anti-Human MUC1 Therapeutic Antibody (TAB-418MZ) in DB

Dot Blot analysis of TAB-418MZ was performed by coating with human MUC1 protein (His tag).

TAB-418MZ incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG was used as a secondary antibody (1: 2000)

Figure 2 Anti-Human MUC1 Therapeutic Antibody (TAB-418MZ) in WB

Western blot analysis of TAB-418MZ was performed with human MUC1 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-418MZ and HRP goat anti-mouse IgG was used as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 3 Mouse Anti-MUC1 Recombinant Antibody (5E5) (TAB-418MZ) in SDS-PAGE

SDS-PAGE analysis of TAB-418MZ in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of TAB-418MZ was greater than 95% as determined by SDS-PAGE

Figure 4 Mouse Anti-MUC1 Recombinant Antibody (5E5) (TAB-418MZ) in HPLC

The purity of TAB-388CT was greater than 93% as determined by SEC-HPLC.