4-1BB (TNFRSF9, CD137, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor)

4-1BB, also known as CD137, is a type I transmembrane protein belonging to the TNF receptor superfamily (TNFRSF9). It is expressed on T cells, NK cells, monocytes, neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell costimulatory signal to promote survival, proliferation, cytokine production, and effector functions. On dendritic cells, 4-1BB ligation enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.

Suitable for use in Flow Cytometry, ELISA, and Western Blot. Other applications not tested.

Flow Cytometry: 2.5ug/10e6 cells
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Mab

IgG2b

9H5

mouse

human

~0.5mg/ml (after reconstitution)

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.

Purified by Protein A affinity chromatography from culture supernatant.

Recombinant protein corresponding to aa24-186 of human 4-1BB expressed in NSO cells. Swiss/UniProt Accession

Recognizes human 4-1BB/TNFRSF9. Does not cross react with recombinant mouse (rm) 4-1BB, recombinant human (rh) CD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLTR beta, rmLTR beta, rhNGFR, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.