MDA (Malondialdehyde) BioAssay™ ELISA Kit

Serum, plasma, tissue homogenates and other biological fluids.

Competitive ELISA, Coated with Antigen

<4.688ng/ml

7.813-500ng/ml

Universal

This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with MDA. During the reaction, MDA in the sample or standard competes with a fixed amount of MDA on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to MDA. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of MDA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Micro ELISA Plate (Dismountable), 8x12
Lyophilized Standard, 2xvial
Sample/Standard dilution buffer, 20ml
Biotin-detection antibody (Concentrated), 120ul
Antibody dilution buffer, 10ml
HRP-Streptavidin Conjugate (SABC), 120ul
SABC dilution buffer, 10ml
TMB substrate, 10ml
Stop solution, 10ml
Wash buffer (25X), 30ml
Plate Sealer, 5xpieces
Product Description, 1xcopy

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

 All products should be handled by qualified personnel only, trained in laboratory procedures.