Malachite Green Phosphate Detection BioAssay™ Kit

The Malachite Green Phosphate Detection Kit is designed for the in vitro measurement of phosphatase activity. Protein phosphatase enzymatic activity results in the release into solution of free inorganic phosphate from protein-phosphatase substrates. This free inorganic phosphate then forms a green molybdophosphoric acid complex. Formation of this complex is monitored and quantified by measuring the absorbance at 630nm. This absorbance is proportional to free-inorganic phosphate in solution and correlates with the activity of phosphatase.||The Malachite Green Phosphate Detection Kit is a convenient and sensitive, single-step free-phosphate determination kit that can be used for measuring phosphate released during enzymatic phosphatase assays.||Detection Range:|50-1600pmol of phosphate||Specificity:|The Malachite Green Phosphate Detection Kit detects only free-phosphate in the sample. ||Test Principle:|The Malachite Green Phosphate Detection BioAssay™ Kit is designed for the in vitro measurement of phosphatase activity. Protein phosphatase enzymatic activity results in the release into solution of free inorganic phosphate from protein-phosphatase substrates. This free inorganic phosphate then forms a green molybdophosphoric acid complex. Formation of this complex is monitored and quantified by measuring the absorbance at 630nm. This absorbance is proportional to free-inorganic phosphate in solution and correlates with the activity of phosphatase.||Kit Components:|351854A: Malachite Green Reagent, 2x28ml|351854B: Phosphate Standard (1mM), 1x500ul||Storage and Stability:|Store all components at RT. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.