Leupeptin (hemisulfate) [103476-89-7]
Katalog-Nummer HY-18234A-5mg
Size : 5mg
Marke : MedChemExpress
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- EN - English
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- Antibody-drug Conjugate/ADC Related
- Apoptosis
- Autophagy
- Cell Cycle/DNA Damage
- Cytoskeleton
- Epigenetics
- GPCR/G Protein
- Immunology/Inflammation
- JAK/STAT Signaling
- MAPK/ERK Pathway
- Membrane Transporter/Ion Channel
- Metabolic Enzyme/Protease
- Neuronal Signaling
- NF-κB
- PI3K/Akt/mTOR
- PROTAC
- Protein Tyrosine Kinase/RTK
- Stem Cell/Wnt
- TGF-beta/Smad
- Vitamin D Related/Nuclear Receptor
- Others
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
- Protocol 1
Add each solvent one by one: PBS
Solubility: 100 mg/mL (210.27 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Dosage
mg/kgAnimal weight
(per animal)
Dosing volume
(per animal)
Number of animals
-
[1]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68. [Content Brief]
[2]. Fu L, et al. Mechanism of Microbial Metabolite Leupeptin in the Treatment of COVID-19 by Traditional Chinese Medicine Herbs. mBio. 2021 Oct 26;12(5):e0222021. [Content Brief]
[3]. Haspel J, et al. Characterization of macroautophagic flux in vivo using a leupeptin-based assay. Autophagy. 2011 Jun;7(6):629-42. [Content Brief]
[4]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68.
-
[1]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68.
[2]. Fu L, et al. Mechanism of Microbial Metabolite Leupeptin in the Treatment of COVID-19 by Traditional Chinese Medicine Herbs. mBio. 2021 Oct 26;12(5):e0222021.
[3]. Haspel J, et al. Characterization of macroautophagic flux in vivo using a leupeptin-based assay. Autophagy. 2011 Jun;7(6):629-42.
[4]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68.
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
---|
H2O | 1 mM | 2.1027 mL | 10.5133 mL | 21.0265 mL | 52.5663 mL |
5 mM | 0.4205 mL | 2.1027 mL | 4.2053 mL | 10.5133 mL | |
10 mM | 0.2103 mL | 1.0513 mL | 2.1027 mL | 5.2566 mL | |
15 mM | 0.1402 mL | 0.7009 mL | 1.4018 mL | 3.5044 mL | |
20 mM | 0.1051 mL | 0.5257 mL | 1.0513 mL | 2.6283 mL | |
25 mM | 0.0841 mL | 0.4205 mL | 0.8411 mL | 2.1027 mL | |
30 mM | 0.0701 mL | 0.3504 mL | 0.7009 mL | 1.7522 mL | |
40 mM | 0.0526 mL | 0.2628 mL | 0.5257 mL | 1.3142 mL | |
50 mM | 0.0421 mL | 0.2103 mL | 0.4205 mL | 1.0513 mL | |
60 mM | 0.0350 mL | 0.1752 mL | 0.3504 mL | 0.8761 mL | |
80 mM | 0.0263 mL | 0.1314 mL | 0.2628 mL | 0.6571 mL | |
100 mM | 0.0210 mL | 0.1051 mL | 0.2103 mL | 0.5257 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
- Metabolic Enzyme/Protease Anti-infection
- Cathepsin Ser/Thr Protease Virus Protease
-
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Leupeptin hemisulfate103476-89-7CathepsinSer/Thr ProteaseVirus ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesMproSARS-CoV-2Vero cellscathepsinserine proteasesthreonine proteasescysteine proteasesInhibitorinhibitorinhibit
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- Leupeptin hemisulfate
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