KLK11 ELISA Kit (Mouse)
Katalog-Nummer OKEH05783
Size : 96Wells
Marke : Aviva Systems Biology
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| Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Klk11 ELISA Kit (Mouse) (OKEH05783) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Klk11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Klk11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Klk11 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 91.8% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <7.9%, n = 20 Mean Inter-Assay Precision - <9.5%, n = 20 | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.078 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Mouse Kallikrein-11 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Klk11 |
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| Gene Full Name | kallikrein related-peptidase 11 |
| Alias Symbols | 2310015I08Rik;hi;hippostasin;kallikrein-11;Pr;Prss20;serine protease 20;T;TLSP. |
| NCBI Gene Id | 56538 |
| Protein Name | Q9QYN3 |
| Description of Target | Kallikrein-11 |
| Uniprot ID | https://www.uniprot.org/uniprot/Q9QYN3 |
| Protein Accession # | NP_001170844.1 |
| Nucleotide Accession # | NM_001177373.1 |
| Protein Size (# AA) | 276 |