Kanamycin Sulfate 50mg/ml Solution
Katalog-Nummer K0015-10x20ml
Size : 10x20ml
Marke : US Biological
Contact local distributor :
Telefonnummer : +1 850 650 7790
Grade
Cell Culture GradeShipping Temp
Blue IceStorage Temp
4°CO-3-Amino-3-deoxy-α-D-glucopyranosyl-(16)-O-[6-amino-6-deoxy-α-D-glucopyranosyl-(14)]-2-deoxy-D-streptamine Sulfate; Cantrex; Cristalomicina; Enterokanacin; Kamycin; Kanabristol; Kanamytrex; Kanaqua; Kanasig; Kanatrol; Kanescin; Otokalixin; Resistomycin; Amikacin EP Impurity D Sulfate
Kanamycin sulfate is an antibiotic that belongs to the group of drugs called aminoglycosides. It binds to ribosomal components and inhibits protein synthesis. Kanamycin sulfate appears to prevent bacteria from making their cell walls; thus the cells die. It is used to treat many sensitive gram-negative, some gram-positive bacteria and and mycoplasmas in cell culture. Kanamycin is inactivated by an aminoglycoside phospho transferase (MW 25,000) in the periplasmic space.
Application:
Suitable for use as a selectable marker for cosmid vectors used in transfection of mammalian cells.
Form:
Filter sterilized (0.2um) solution in 0.9% saline. Supplied in 20ml amber screw-cap vials.
pH:
6.5-8.5
Appearance:
Clear solution
Chromatographic Purity:
To pass USP
Identity:
To pass USP
Activity:
≥750ug/mg
Crystallinity:
To pass USP
Specific Rotation:
+107° to +121°
Endotoxin:
≤2Eu/mg
Working Concentration:
Cell/Tissue Culture: ~30ug/ml
Stringent Plasmids: 10ug/ml
Relaxed Plasmids: 50ug/ml
Stringent Plasmids: 10ug/ml
Relaxed Plasmids: 50ug/ml
Storage and Stability:
Store liquid at 4°C. Stable for for 12 months at 4°C. Further dilutions can be made in assay buffer. Stability at 37°C: 5 days.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards