Isoliquiritigenin is an anti-tumor flavonoid from the root of Glycyrrhiza uralensis Fisch., which inhibits aldose reductase with an IC50 of 320 nM. Isoliquiritigenin is a potent inhibitor of influenza virus replication with an EC50 of 24.7 μM.
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Isoliquiritigenin Chemische Struktur
CAS. Nr. : 961-29-5
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Based on 21 publication(s) in Google Scholar
Isoliquiritigenin purchased from MedChemExpress. Usage Cited in:
Onco Targets Ther. 2018 Mar 22;11:1633-1642.
[Abstract]
Isoliquiritigenin (ILQ) treatment affects the expression level of apoptosis-related proteins.
Isoliquiritigenin purchased from MedChemExpress. Usage Cited in:
Chemotherapy. 2018 Jun 22;63(3):155-161.
[Abstract]
Western blot analysis of the effect on Bcl2, Bax, and active Caspase-3 protein expression in Isoliquiritigenin (ISL) treated U2OS cells.
Isoliquiritigenin purchased from MedChemExpress. Usage Cited in:
Oncol Lett. 2018 Nov;16(5):6133-6139.
[Abstract]
Western blot analysis of the expression of E cadherin, N cadherin and vimentin in A549 cells following treatment with Isoliquiritigenin (ISL) for 48 h.
Isoliquiritigenin purchased from MedChemExpress. Usage Cited in:
Am J Transl Res. 2018 Dec 15;10(12):4141-4151.
[Abstract]
ISL inhibits phosphorylation of IκB-α following LPS stimulation. The expressions of IκB-α and p-IκB are measured by western blot. The samples are harvested from cells and mice. The results show that ISL could effectively decrease protein levels of p-IκB in HK2 of the LPS-induced mice but increase IκB-α.
Isoliquiritigenin purchased from MedChemExpress. Usage Cited in:
Am J Transl Res. 2018 Dec 15;10(12):4141-4151.
[Abstract]
The expression of CD68, CD206, iNOS, MPO is determined by immunohistochemistry in four animal groups. LPS increases the expression of CD68, iNOS and MPO but decreases the expression of CD206 in murine kidney, while ISL reduces the expression of CD68, iNOS and MPO then increases the expression of CD206 in mice kidney after LPS injection.
Western blot assays detect apoptosis-related protein expression of Bax, Bcl-2, active caspase-3.
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Beschreibung
Isoliquiritigenin is an anti-tumor flavonoid from the root of Glycyrrhiza uralensis Fisch., which inhibits aldose reductase with an IC50 of 320 nM. Isoliquiritigenin is a potent inhibitor of influenza virus replication with an EC50 of 24.7 μM.
IC50 & Target
IC50: 320 nM (Aldose reductase)
In Vitro
Isoliquiritigenin may prevent diabetic complications through inhibiting rat lens aldose reductase with IC50=320 nM and sorbitol accumulation in human red blood cells with IC50=2.0 μM[1]. Isoliquiritigenin (100 μM) markedly inhibits the hypoxia-induced prolonged TPS and TR90 of cardiomyocytes. Isoliquiritigenin significantly triggers AMPK Thr172 phosphorylation as compared with vehicle group. Isoliquiritigenin treatment also induces extracellular signal-regulated kinase (ERK) signaling pathway in the cardiomyocytes. Isoliquiritigenin treatment significantly decreases the intracellular ROS levels of isolated cardiomyocytes during hypoxia/reoxygenation[3]. Isoliquiritigenin not only downregulates IL-6 expression but also significantly decreases levels of phosphorylated ERK and STAT3 and can inhibit phosphorylation levels of ERK and STAT3 induced by recombinant human IL-6, which are critical signaling proteins in IL-6 signaling regulation networks[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Isoliquiritigenin Related Antibodies
In Vivo
Isoliquiritigenin shows concentration-dependent inhibition of the tonic contraction of mouse jejunum induced by various types of stimulants such as CCh (1 mM), KCl (60 mM) and BaCl2 (0.3 mM) with IC50 values of 4.96±1.97 mM, 4.03±1.34 mM and 3.70±0.58 mM, respectively[2]. Isoliquiritigenin exhibits significant anti-tumor activity in MM xenograft models and synergistically enhances the anti-myeloma activity of adriamycin[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molekulargewicht
256.25
Formel
C15H12O4
CAS. Nr.
961-29-5
Appearance
Solid
Color
Light yellow to yellow
SMILES
O=C(C1=CC=C(O)C=C1O)/C=C/C2=CC=C(O)C=C2
Structure Classification
Flavonoids
Chalcones
Phenols
Polyphenols
Initial Source
Plants
Leguminosae
Glycyrrhiza uralensis Fisch.
Microorganisms
Versand
Room temperature in continental US; may vary elsewhere.
Speicherung
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
1 year
-20°C
6 months
Lösungsmittel & Löslichkeit
In Vitro:
Ethanol : 100 mg/mL (390.24 mM; Need ultrasonic)
DMSO : ≥ 100 mg/mL (390.24 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
*"≥" means soluble, but saturation unknown.
Preparing Stock Solutions
ConcentrationSolventMass
1 mg
5 mg
10 mg
1 mM
3.9024 mL
19.5122 mL
39.0244 mL
5 mM
0.7805 mL
3.9024 mL
7.8049 mL
10 mM
0.3902 mL
1.9512 mL
3.9024 mL
View the Complete Stock Solution Preparation Table
*Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Protocol 1
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μLDMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μLDMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3
Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μLDMSO stock solution (25.0 mg/mL) to 900 μLCorn oil, and mix evenly.
Protocol 4
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 6
Add each solvent one by one: 10% EtOH 90% Corn Oil
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μLCorn oil, and mix evenly.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage
mg/kg
Animal weight (per animal)
g
Dosing volume (per animal)
μL
Number of animals
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO+
%
+
%
Tween-80
+
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
[1]. Aida K, et al. Isoliquiritigenin: a new aldose reductase inhibitor from glycyrrhizae radix. Planta Med. 1990 Jun;56(3):254-8.
[Content Brief]
[2]. Sato Y, et al. Isoliquiritigenin, one of the antispasmodic principles of Glycyrrhiza ularensis roots, acts in the lower part of intestine. Biol Pharm Bull. 2007 Jan;30(1):145-9.
[Content Brief]
[3]. Zhang X. Natural antioxidant-isoliquiritigenin ameliorates contractile dysfunction of hypoxic cardiomyocytes via AMPK signaling pathway. Mediators Inflamm. 2013;2013:390890.
[Content Brief]
[4]. Chen X, et al. Isoliquiritigenin inhibits the growth of multiple myeloma via blocking IL-6 signaling. J Mol Med (Berl). 2012 Nov;90(11):1311-9.
[Content Brief]
[5]. Traboulsi H, et al. The Flavonoid Isoliquiritigenin Reduces Lung Inflammation and Mouse Morbidity during Influenza Virus Infection. Antimicrob Agents Chemother. 2015 Oct;59(10):6317-27.
[Content Brief]
[1]. Aida K, et al. Isoliquiritigenin: a new aldose reductase inhibitor from glycyrrhizae radix. Planta Med. 1990 Jun;56(3):254-8.
[2]. Sato Y, et al. Isoliquiritigenin, one of the antispasmodic principles of Glycyrrhiza ularensis roots, acts in the lower part of intestine. Biol Pharm Bull. 2007 Jan;30(1):145-9.
[3]. Zhang X. Natural antioxidant-isoliquiritigenin ameliorates contractile dysfunction of hypoxic cardiomyocytes via AMPK signaling pathway. Mediators Inflamm. 2013;2013:390890.
[4]. Chen X, et al. Isoliquiritigenin inhibits the growth of multiple myeloma via blocking IL-6 signaling. J Mol Med (Berl). 2012 Nov;90(11):1311-9.
[5]. Traboulsi H, et al. The Flavonoid Isoliquiritigenin Reduces Lung Inflammation and Mouse Morbidity during Influenza Virus Infection. Antimicrob Agents Chemother. 2015 Oct;59(10):6317-27.
Complete Stock Solution Preparation Table
*Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Optional Solvent
ConcentrationSolventMass
1 mg
5 mg
10 mg
25 mg
Ethanol / DMSO
1 mM
3.9024 mL
19.5122 mL
39.0244 mL
97.5610 mL
5 mM
0.7805 mL
3.9024 mL
7.8049 mL
19.5122 mL
10 mM
0.3902 mL
1.9512 mL
3.9024 mL
9.7561 mL
15 mM
0.2602 mL
1.3008 mL
2.6016 mL
6.5041 mL
20 mM
0.1951 mL
0.9756 mL
1.9512 mL
4.8780 mL
25 mM
0.1561 mL
0.7805 mL
1.5610 mL
3.9024 mL
30 mM
0.1301 mL
0.6504 mL
1.3008 mL
3.2520 mL
40 mM
0.0976 mL
0.4878 mL
0.9756 mL
2.4390 mL
50 mM
0.0780 mL
0.3902 mL
0.7805 mL
1.9512 mL
60 mM
0.0650 mL
0.3252 mL
0.6504 mL
1.6260 mL
80 mM
0.0488 mL
0.2439 mL
0.4878 mL
1.2195 mL
100 mM
0.0390 mL
0.1951 mL
0.3902 mL
0.9756 mL
Isoliquiritigenin Related Classifications
Help & FAQs
Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
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