Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Immunofluorescence (IC): 1:1600
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Mab

IgG1,k

2D2-B2

mouse

human

Not Determined

Supplied as a liquid

Ascites

Recombinant protein corresponding to human Inducible Nitric Oxide Synthase

Recognizes human iNOS (781-798) and rhiNOS (Type II). Does not react to heNOS (806-823), rheNOS (Type III), hnNOS (1045-1062), rhnNOS (Type I). It has been epitope mapped using 96 overlapping 18 amino acid synthetic peptides which cover the entire 1153 amino acid length of hiNOS and was found to bind to a defined region of the hiNOS sequence, residues 781-798.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.