Indole-3-butyric acid conjugated to BSA

Background
Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings. According to new EC regulations, IBA levels should approach zero at the time the plantlets are entering the market. In order to be able to measure IBA in soil samples, polyclonal antibodies and a corresponding ELISA was developed. The highly purified BSA-Indole-3-butyric acid conjugate can be used as a standard in antibody-based tests.

Product
Each vial contains 100µg 1mg/ml BSA-Indole-3-butyric acid conjugate in PBS, pH 7.4. The conjugate was purified and isolated using a Biorad Econo-Pac 10DG column with HSB as mobile phase according to the recommendations of the manufacturer.

Applications
The BSA-Indole-3-butyric acid conjugate is suitable as a standard for use in Western blotting and ELISA. Optimal protein dilutions for the different applications should be determined by titration.

Storage
Maintain at -20°C in undiluted aliquots for up to 24 months after date of receipt. Avoid repeated freeze-thaw cycles.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
Meulenberg EP (2015) The development and application of ELISA for 3-Indole-butyric acid. Immunochem Immunopathol: Open Access 1: 1000104.