AP6284c | INA (alpha internexin) Antibody (Center) Biotrend

All lanes : Anti-INA Antibody (Center) at 1:1000-2000 dilution Lane 1: Human brain tissue lysate Lane 2: U-87 MG whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-INA Antibody (Center) at 1:2000 dilution Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody (Center) (Cat.#AP6284c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	IHC-P, WB, E
Primary Accession	Q16352
Other Accession	P23565, P46660, Q08DH7
Reactivity	Human, Mouse, Rat
Predicted	Bovine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	55391 Da
Antigen Region	290-319 aa

Additional Information
Gene ID	9118
Other Names	Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5
Target/Specificity	This INA (alpha internexin) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from the Central region of human INA (alpha internexin).
Dilution	WB~~1:2000 IHC-P~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	INA (alpha internexin) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	INA
Synonyms	NEF5
Function	Class-IV neuronal intermediate filament that is able to self- assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity).
Tissue Location	Found predominantly in adult CNS.

INA (alpha internexin) Antibody (Center)