IL-10 (Interleukin-10) BioAssay™ ELISA Kit (Hamster)

Serum, plasma, tissue homogenates and other biological fluids.

Sandwich ELISA, Double Antibody

<18.75pg/ml

31.2-2000pg/ml

Human

This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SSB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SSB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SSB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SSB can be calculated.

Micro ELISA Plate (Dismountable), 8x12
Lyophilized Standard, 2xvial
Sample/Standard dilution buffer, 20ml
Biotin-detection antibody (Concentrated), 120ul
Antibody dilution buffer, 10ml
HRP-Streptavidin Conjugate (SABC), 120ul
SABC dilution buffer, 10ml
TMB substrate, 10ml
Stop solution, 10ml
Wash buffer (25X), 30ml
Plate Sealer, 5xpieces
Product Description, 1xcopy

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hamster

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.