Human NRG1 (Neuregulin 1) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	NRG1
Gene ID:	3084
Uniprot ID:	NRG1_HUMAN
Specificity:	This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
Function	Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells.inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction.stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells.stimulating Schwann cell proliferation.implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4. Binds to ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons.
Protein Name	Pro-Neuregulin-1 - Membrane-Bound Isoform Pro-Nrg1 Cleaved Into - Neuregulin-1 Acetylcholine Receptor-Inducing Activity Aria Breast Cancer Cell Differentiation Factor P45 Glial Growth Factor Heregulin Hrg Neu Differentiation Factor Sensory And Motor Neuron-Derived Factor
Database Links	Reactome: R-HSA-1227986 Q02297-10 Reactome: R-HSA-1236394 Q02297-10 Reactome: R-HSA-1250196 Q02297-10 Reactome: R-HSA-1250342 Reactome: R-HSA-1250347 Reactome: R-HSA-1251985 Reactome: R-HSA-1257604 Q02297-10 Reactome: R-HSA-1306955 Q02297-10 Reactome: R-HSA-1358803 Q02297-10 Reactome: R-HSA-1963640 Reactome: R-HSA-1963642 Q02297-10 Reactome: R-HSA-2219530 Q02297-10 Reactome: R-HSA-5673001 Q02297-10 Reactome: R-HSA-6785631 Q02297-10 Reactome: R-HSA-6811558 Q02297-10 Reactome: R-HSA-8847993 Q02297-10 Reactome: R-HSA-8863795 Q02297-10 Reactome: R-HSA-9620244 Reactome: R-HSA-9664565 Q02297-10 Reactome: R-HSA-9665686 Q02297-10
Cellular Localisation	Pro-Neuregulin-1 Membrane-Bound Isoform: Cell Membrane Single-Pass Type I Membrane Protein Does Not Seem To Be Active Neuregulin-1: Secreted Isoform 8: Nucleus May Be Nuclear Isoform 9: Secreted Has A Signal Peptide Isoform 10: Membrane May Possess An Internal Uncleaved Signal Sequence
Alternative ELISA Names	Pro-Neuregulin-1 - Membrane-Bound Isoform ELISA kit Pro-Nrg1 Cleaved Into - Neuregulin-1 ELISA kit Acetylcholine Receptor-Inducing Activity ELISA kit Aria ELISA kit Breast Cancer Cell Differentiation Factor P45 ELISA kit Glial Growth Factor ELISA kit Heregulin ELISA kit Hrg ELISA kit Neu Differentiation Factor ELISA kit Sensory And Motor Neuron-Derived Factor ELISA kit NRG1 ELISA kit GGF ELISA kit HGL ELISA kit HRGA ELISA kit NDF ELISA kit SMDF ELISA kit
Specificity	This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-97	90
EDTA plasma (n=8)	97-110	104
Cell culture media (n=8)	92-104	99

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	49.09	112.92	495.58	45.27	108.53	485.65
Standard deviation	2.76	5.33	23.79	2.72	5.21	21.32
CV (%)	5.62	4.72	4.80	6.01	4.80	4.39

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance