Specifications

Product Data
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	2.6 -66 pmol/l
Sample Type	Serum
Sample Volume	25µl/well
Specificity	This kit is used for quantitative detection human Insulin
Sensitivity	2.6 pmol/l
Reactivities	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwell coated with Streptavidin: 12x8x1 2. Insulin Standards: 6 vials ( ready to use): 0.5ml 3. Insulin Conjugate reagent: 1 vial: 12 ml 4. TMB Substrate: 1 bottle (ready to use): 12ml 5. Stop Solution: 1 bottle (ready to use): 12ml 6. 20X Wash concentrate: 1 bottle: 25ml
Background	Insulin is the principal hormone responsible for the control of glucose metabolism. It is synthesized in the β- cells of the islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin. Both are secreted in equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chains, the A chain and B chain (21 and 30 amino acids respectively). The two chains are linked together by two inter-chain disulphide bridges. There is also an intra-chain disulphide bridge in the A chain. Insulin concentrations are severely reduced in insulin-dependent diabetes mellitus (IDDM) and some other conditions such as hypopituitarism. Insulin levels are raised in non-insulin- dependent diabetes mellitus (NIDDM), obesity, insulinoma and some endocrine dysfunctions such as Cushing’s syndrome and acromegaly.