Human COL4a3 (Collagen Type IV Alpha 3) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	COL4A3
Gene ID:	1285
Uniprot ID:	CO4A3_HUMAN
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.
Post Translational Modifications	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Isoform 2 contains an additional N-linked glycosylation site. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. Phosphorylated. Thought to be phosphorylated by CERT, but CERT does not have kinase activity.
Function	Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.
Protein Name	Collagen Alpha-3(Iv Chain Goodpasture Antigen Cleaved Into - Tumstatin
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-186797 Reactome: R-HSA-2022090 Reactome: R-HSA-216083 Reactome: R-HSA-2214320 Reactome: R-HSA-2243919 Reactome: R-HSA-3000157 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-419037 Reactome: R-HSA-8948216
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix Basement Membrane Colocalizes With Col4a4 And Col4a5 In Gbm Tubular Basement Membrane (Tbm) And Synaptic Basal Lamina (Bl)
Alternative ELISA Names	Collagen Alpha-3(Iv Chain ELISA kit Goodpasture Antigen Cleaved Into - Tumstatin ELISA kit COL4A3 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance