General Info
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69pg/mL |
| Detection Limit: | 7.81~500pg/mL |
| Short Description: | This CCL19 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
Information
| Gene Symbol: | CCL19 |
| Gene ID: | 6363 |
| Uniprot ID: | CCL19_HUMAN |
| Specificity: | This kit recognizes Human MIP-3 Beta/ELC/CCL19 in samples. No significant cross-reactivity or interference between Human MIP-3 Beta/ELC/CCL19 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Function | May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. |
| Protein Name | C-C Motif Chemokine 19Beta-Chemokine Exodus-3Ck Beta-11Epstein-Barr Virus-Induced Molecule 1 Ligand ChemokineEbi1 Ligand ChemokineElcMacrophage Inflammatory Protein 3 BetaMip-3-BetaSmall-Inducible Cytokine A19 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 19 ELISA kitBeta-Chemokine Exodus-3 ELISA kitCk Beta-11 ELISA kitEpstein-Barr Virus-Induced Molecule 1 Ligand Chemokine ELISA kitEbi1 Ligand Chemokine ELISA kitElc ELISA kitMacrophage Inflammatory Protein 3 Beta ELISA kitMip-3-Beta ELISA kitSmall-Inducible Cytokine A19 ELISA kitCCL19 ELISA kitELC ELISA kitMIP3B ELISA kitSCYA19 ELISA kit |
| Specificity | This kit recognizes Human MIP-3β/ELC/CCL19 in samples. No significant cross-reactivity or interference between Human MIP-3β/ELC/CCL19 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-99 | 95 |
| EDTA plasma (n=8) | 94-104 | 99 |
| Cell culture media (n=8) | 94-109 | 102 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 22.52 | 53.02 | 228.89 | 22.80 | 62.03 | 249.79 |
| Standard deviation | 1.21 | 2.57 | 9.29 | 1.22 | 2.84 | 10.12 |
| CV (%) | 5.37 | 4.85 | 4.06 | 5.35 | 4.58 | 4.05 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance