Human CALR (Calreticulin) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	CALR
Gene ID:	811
Uniprot ID:	CALR_HUMAN
Specificity:	This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Protein Name	Calreticulin Crp55 Calregulin Endoplasmic Reticulum Resident Protein 60 Erp60 Hacbp Grp60
Database Links	Reactome: R-HSA-1236974 Reactome: R-HSA-168316 Reactome: R-HSA-3000480 Reactome: R-HSA-3000484 Reactome: R-HSA-381183 Reactome: R-HSA-901042 Reactome: R-HSA-983170
Cellular Localisation	Endoplasmic Reticulum Lumen Cytoplasm Cytosol Secreted Extracellular Space Extracellular Matrix Cell Surface Sarcoplasmic Reticulum Lumen Cytoplasmic Vesicle Secretory Vesicle Cortical Granule Cytolytic Granule Also Found In Cell Surface (T Cells) Cytosol And Extracellular Matrix During Oocyte Maturation And After Parthenogenetic Activation Accumulates In Cortical Granules In Pronuclear And Early Cleaved Embryos Localizes Weakly To Cytoplasm Around Nucleus And More Strongly In The Region Near The Cortex In Cortical Granules Of Non-Activated Oocytes Is Exocytosed During The Cortical Reaction In Response To Oocyte Activation
Alternative ELISA Names	Calreticulin ELISA kit Crp55 ELISA kit Calregulin ELISA kit Endoplasmic Reticulum Resident Protein 60 ELISA kit Erp60 ELISA kit Hacbp ELISA kit Grp60 ELISA kit CALR ELISA kit CRTC ELISA kit
Specificity	This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-105	99
EDTA plasma (n=8)	86-98	91
Cell culture media (n=8)	90-105	96

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.53	1.36	4.94	0.52	1.31	4.68
Standard deviation	0.03	0.06	0.26	0.03	0.06	0.16
CV (%)	5.66	4.41	5.26	5.77	4.58	3.42

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance