Human BRCA2 (Breast Cancer Susceptibility Protein 2) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	BRCA2
Gene ID:	675
Uniprot ID:	BRCA2_HUMAN
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
Post Translational Modifications	Phosphorylated by ATM upon irradiation-induced DNA damage. Phosphorylation by CHEK1 and CHEK2 regulates interaction with RAD51. Phosphorylation at Ser-3291 by CDK1 and CDK2 is low in S phase when recombination is active, but increases as cells progress towards mitosis.this phosphorylation prevents homologous recombination-dependent repair during S phase and G2 by inhibiting RAD51 binding. Ubiquitinated in the absence of DNA damage.this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.
Function	Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks.together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination.
Protein Name	Breast Cancer Type 2 Susceptibility Protein Fanconi Anemia Group D1 Protein
Database Links	Reactome: R-HSA-5685939 Reactome: R-HSA-5685942 Reactome: R-HSA-5693554 Reactome: R-HSA-5693568 Reactome: R-HSA-5693579 Reactome: R-HSA-5693616 Reactome: R-HSA-912446 Reactome: R-HSA-9701192 Reactome: R-HSA-9704331 Reactome: R-HSA-9704646 Reactome: R-HSA-9709275 Reactome: R-HSA-9709570 Reactome: R-HSA-9709603 Reactome: R-HSA-9763198
Cellular Localisation	Nucleus Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Colocalizes With Ercc5/Xpg To Nuclear Foci Following Dna Replication Stress
Alternative ELISA Names	Breast Cancer Type 2 Susceptibility Protein ELISA kit Fanconi Anemia Group D1 Protein ELISA kit BRCA2 ELISA kit FACD ELISA kit FANCD1 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance