Human BLK (B-Lymphoid Tyrosine Kinase) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	BLK
Gene ID:	640
Uniprot ID:	BLK_HUMAN
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
Post Translational Modifications	Phosphorylated on tyrosine residues after antibody-mediated surface engagement of the B-cell antigen receptor (BCR). Ubiquitination of activated BLK by the UBE3A ubiquitin protein ligase leads to its degradation by the ubiquitin-proteasome pathway.
Function	Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Phosphorylates CGAS, promoting retention of CGAS in the cytosol.
Protein Name	Tyrosine-Protein Kinase Blk B Lymphocyte Kinase P55-Blk
Database Links	Reactome: R-HSA-8939245 Reactome: R-HSA-983695
Cellular Localisation	Cell Membrane Lipid-Anchor Present And Active In Lipid Rafts Membrane Location Is Required For The Phosphorylation Of Cd79a And Cd79b
Alternative ELISA Names	Tyrosine-Protein Kinase Blk ELISA kit B Lymphocyte Kinase ELISA kit P55-Blk ELISA kit BLK ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance