Human BAX (Apoptosis regulator BAX) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	BAX
Gene ID:	581
Uniprot ID:	BAX_HUMAN
Specificity:	This kit recognizes Human BAX in samples. No significant cross-reactivity or interference between Human BAX and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12.
Function	Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Protein Name	Apoptosis Regulator Bax Bcl-2-Like Protein 4 Bcl2-L-4
Database Links	Reactome: R-HSA-111457 Reactome: R-HSA-114294 Reactome: R-HSA-5620971 Reactome: R-HSA-6803204 Reactome: R-HSA-6804114 Reactome: R-HSA-8878166 Reactome: R-HSA-9603505
Cellular Localisation	Isoform Alpha: Mitochondrion Outer Membrane Single-Pass Membrane Protein Cytoplasm Nucleus Colocalizes With 14-3-3 Proteins In The Cytoplasm Under Stress Conditions Undergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion Membrane Upon Sendai Virus Infection Recruited To The Mitochondrion Through Interaction With Irf3 Isoform Beta: Cytoplasm Isoform Gamma: Cytoplasm Isoform Delta: Cytoplasm
Alternative ELISA Names	Apoptosis Regulator Bax ELISA kit Bcl-2-Like Protein 4 ELISA kit Bcl2-L-4 ELISA kit BAX ELISA kit BCL2L4 ELISA kit
Specificity	This kit recognizes Human BAX in samples. No significant cross-reactivity or interference between Human BAX and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-106	99
EDTA plasma (n=8)	88-102	93
Cell culture media (n=8)	89-104	96

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	2.37	4.27	18.72	2.53	4.30	17.45
Standard deviation	0.15	0.24	0.88	0.13	0.19	0.58
CV (%)	6.33	5.62	4.70	5.14	4.42	3.32

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance