General Info
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.47ng/mL |
| Detection Limit: | 0.78~50ng/mL |
| Short Description: | This APOF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
Information
| Gene Symbol: | APOF |
| Gene ID: | 319 |
| Uniprot ID: | APOF_HUMAN |
| Specificity: | This kit recognizes Human ApoF in samples. No significant cross-reactivity or interference between Human ApoF and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Post Translational Modifications | O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII. |
| Protein Name | Apolipoprotein FApo-FLipid Transfer Inhibitor ProteinLtip |
| Database Links | Reactome: R-HSA-8964041 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Apolipoprotein F ELISA kitApo-F ELISA kitLipid Transfer Inhibitor Protein ELISA kitLtip ELISA kitAPOF ELISA kit |
| Specificity | This kit recognizes Human ApoF in samples. No significant cross-reactivity or interference between Human ApoF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 82-93 | 90 |
| EDTA plasma (n=8) | 94-103 | 100 |
| Cell culture media (n=8) | 91-101 | 95 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.17 | 5.23 | 23.43 | 2.42 | 5.05 | 22.81 |
| Standard deviation | 0.12 | 0.26 | 1.25 | 0.13 | 0.25 | 1.02 |
| CV (%) | 5.53 | 4.97 | 5.34 | 5.37 | 4.95 | 4.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance