Human APOC2 (Apolipoprotein C-II) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	APOC2
Gene ID:	344
Uniprot ID:	APOC2_HUMAN
Specificity:	This kit recognizes Human ApoC2 in samples. No significant cross-reactivity or interference between Human ApoC2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Proapolipoprotein C-II is synthesized as a sialic acid containing glycoprotein which is subsequently desialylated prior to its proteolytic processing. Proapolipoprotein C-II, the major form found in plasma undergoes proteolytic cleavage of its N-terminal hexapeptide to generate apolipoprotein C-II, which occurs as the minor form in plasma.
Function	Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.
Protein Name	Apolipoprotein C-Ii Apo-Cii Apoc-Ii Apolipoprotein C2 Cleaved Into - Proapolipoprotein C-Ii Proapoc-Ii
Database Links	Reactome: R-HSA-8963888 Reactome: R-HSA-8963889 Reactome: R-HSA-8963901 Reactome: R-HSA-8964058 Reactome: R-HSA-9029569 Reactome: R-HSA-975634
Cellular Localisation	Secreted
Alternative ELISA Names	Apolipoprotein C-Ii ELISA kit Apo-Cii ELISA kit Apoc-Ii ELISA kit Apolipoprotein C2 Cleaved Into - Proapolipoprotein C-Ii ELISA kit Proapoc-Ii ELISA kit APOC2 ELISA kit APC2 ELISA kit
Specificity	This kit recognizes Human ApoC2 in samples. No significant cross-reactivity or interference between Human ApoC2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-97	90
EDTA plasma (n=8)	90-102	97
Cell culture media (n=8)	86-96	91

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	11.00	19.50	83.60	12.10	19.90	86.30
Standard deviation	0.80	0.80	3.90	0.80	0.90	2.90
CV (%)	7.27	4.10	4.67	6.61	4.52	3.36

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance