HSPA5 Antibody
Katalog-Nummer OAED00189
Size : 50ug
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
HSPA5 Antibody (OAED00189)
| Datasheets/Manuals | Printable datasheet for HSPA5 Antibody (OAED00189) |
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| Predicted Species Reactivity | Bovine|Fungus|Hamster|Monkey|Mouse|Rabbit|Rat|Xenopus |
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| Product Format | Liquid PBS containing 50% glycerol and 0.09% sodium azide. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | Western blot |
| Reconstitution and Storage | Long Term Storage : -20°C. Avoid freeze/thaw cycles. |
| Immunogen | Synthetic peptide corresponding to the sequence near the C-terminus of rat Grp78 (BiP). The sequence is completely conserved in mouse and hamster. |
| Purification | Protein A affinity purified. |
| Concentration | 1 mg/ml |
| Specificity | Detects a band of ~78kDa by Western blot. |
| Application Info | WB (ECL): 1:1000 |
| Reference | 1. ERp29, an Endoplasmic Reticulum Secretion Factor Is Involved in the Growth of Breast Tumor Xenografts: S. Mkrtchian, et al. ; Mol. Carcinog. 47, 886 (2008), Application(s): WB using human tissue culture, Abstract; 2. Y Nerve growth factor blocks thapsigargin-induced apoptosis at the level of the mitochondrion via regulation of Bim: A. Samali, et al. ; J. Cell. Mol. Med. 12, 2482 (2008), Application(s): WB using rat cell lysates, Abstract; 3. Small heat-shock proteins select &UDelta;F508-CFTR for endoplasmic reticulum-associated degradation: J. Brodsky, et al. ; Mol. Biol. Cell 18, 806 (2007), Application(s): WB using human cell lysates, Abstract; 4. Bax plays a pivotal role in thapsigargin-induced apoptosis of human colon cancer HCT116 cells by controlling Smac/Diablo and Omi/HtrA2 release from mitochondria: H.G. Wang, et al. ; Cancer Res. 63, 1483 (2003), Application(s): WB using human samples, Abstract; 5. gamma-Secretase activity requires the presenilin-dependent trafficking of nicastrin through the Golgi apparatus but not its complex glycosylation: B. De Strooper, et al. ; J. Cell. Sci. 116, 1127 (2003), Application(s): ICC using mouse samples, Abstract; 6. Mutant SOD1 linked to familial amyotrophic lateral sclerosis, but not wild-type SOD1, induces ER stress in COS7 cells and transgenic mice: T. Kato, et al. ; Biochem. Biophys. Res. Commun. 303, 496 (2003), Application(s): ICC, WB, IHC using monkey samples, Abstract; 7. Differential interaction of molecular chaperones with procollagen I and type IV collagen in corneal endothelial cells: E.P. Kay, et al. ; Mol. Vis. 8, 9 (2002), Application(s): ICC using rabbit samples, Abstract; 8. Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP: M. Mori, et al. ; J. Biol. Chem. 277, 12343 (2002), Application(s): WB using mouse samples, Abstract; 9. The protein kinase/endoribonuclease IRE1 alpha that signals the unfolded protein response has a luminal N-terminal ligand-independent dimerization domain: R.J. Kaufman, et al. ; J. Biol. Chem. 277, 18346 (2002), Application(s): IP using human & monkey samples, Abstract; 10. Truncation releases olfactory receptors from the endoplasmic reticulum of heterologous cells: T.S. McClintock, et al. ; J. Neurochem. 72, 2301 (1999), Application(s): ICC using human & xenopus samples, Abstract; |
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| Gene Symbol | Hspa5 |
| Gene Full Name | heat shock protein family A (Hsp70) member 5 |
| Alias Symbols | 78 kDa glucose-regulated protein;binding-immunoglobulin protein;BIP;endoplasmic reticulum chaperone BiP;GRP 78;GRP78;GRP-78;heat shock 70 kDa protein 5;heat shock 70kD protein 5;heat shock 70kDa protein 5 (glucose-regulated protein);heat shock protein 5;heat shock protein 70 family protein 5;heat shock protein family A member 5;HSP70 family protein 5;immunoglobulin heavy chain-binding protein;steroidogenesis-activator polypeptide. |
| NCBI Gene Id | 25617 |
| Protein Name | Endoplasmic reticulum chaperone BiP |
| Description of Target | Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (By similarity). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). |
| Uniprot ID | P06761 |
| Protein Accession # | NP_005338.1 |
| Nucleotide Accession # | NM_005347.4 |
