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HSF2 Antibody

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Katalog-Nummer ASM10030-STR-100

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Marke : Abcepta

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  • ICC/IF - HSF2 Antibody ASM10030
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (ASM10030). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (ASM10030) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite.
  • WB - HSF2 Antibody ASM10030
    Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (ASM10030). Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (ASM10030) at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.
  • ICC/IF - HSF2 Antibody ASM10030
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (ASM10030). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (ASM10030) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, AM
Primary Accession P38533 
Other Accession NP_001129036.1
Host Rat
Isotype IgG
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Bovine, Sheep, Guinea Pig, Dog
Clonality Monoclonal
Description Rat Anti-Mouse HSF2 Monoclonal IgG
Target/Specificity Detects ~69kDa.
Other Names HSTF2 Antibody, Heat shock factor protein 2 Antibody, Heat shock transcription factor 2 Antibody, HSF 2 Antibody
Clone Names 3E2
Immunogen Purified recombinant mouse HSF2 protein
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 4 µg/ml of SMC-119 was sufficient for detection of HSF2 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus