Specifications

Product Data
Applications	ELISA, FC, ICC/IF, IHC, Simple Western, WB
Recommended Dilution	Simple Western: 1:2000, Flow Cytometry: 1:100, Flow (Intracellular), ELISA, Western Blot: 0.5-1.0 ug/ml, Immunocytochemistry/ Immunofluorescence: 0.05 ug/ml, Immunohistochemistry: 1:100-1:250, Immunohistochemistry-Paraffin: 1:100-1:250, Knockdown Validated
Reactivities	Human, Mouse, Dog, Bovine, Hamster, Rat
Host	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]
Formulation	Tris-glycine, 150mM NaCl and 0.1% sodium azide
Purification	Affinity purified
Conjugation	Unconjugated
Storage	Store at -20°C as received.
Stability	Stable for 12 months from date of receipt.
Gene Name	high mobility group box 1
Database Link	NP_002119 Entrez Gene 15289 MouseEntrez Gene 25459 RatEntrez Gene 403170 DogEntrez Gene 3146 Human UniProt ID P09429
Background	HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. These proteins (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. They may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.
Synonyms	HMG1; HMG3; SBP-1
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Base excision repair