GSDMA (NM_178171) Human Recombinant Protein
CAT#: TP319050
Recombinant protein of human gasdermin A (GSDMA), 20 µg
Size: 100 ug 1 mg
Product Images
Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | >RC219050 protein sequence Red=Cloning site Green=Tags(s) MTMFENVTRALARQLNPRGDLTPLDSLIDFKRFHPFCLVLRKRKSTLFWGARYVRTDYTLLDVLEPGSSP SDPTDTGNFGFKNMLDTRVEGDVDVPKTVKVKGTAGLSQNSTLEVQTLSVAPKALETLQERKLAADHPFL KEMQDQGENLYVVMEVVETVQEVTLERAGKAEACFSLPFFAPLGLQGSINHKEAVTIPKGCVLAFRVRQL MVKGKDEWDIPHICNDNMQTFPPGEKSGEEKVILIQASDVGDVHEGFRTLKEEVQRETQQVEKLSRVGQS SLLSSLSKLLGKKKELQDLELALEGALDKGHEVNLEALPKDVLLSKEAVGAILYFVGALTELSEAQQKLL VKSMEKKILPVQLKLVESTMEQNFLLDKEGVFPLQPELLSSLGDEELTLTEALVGLSGLEVQRSGPQYMW DPDTLPRLCALYAGLSLLQQLTKAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-Myc/DDK |
| Predicted MW | 49.2 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Reference Data | |
| RefSeq | NP_835465 |
| Locus ID | 284110 |
| UniProt ID | Q96QA5 |
| Cytogenetics | 17q21.1 |
| Refseq Size | 2164 |
| Refseq ORF | 1335 |
| Synonyms | FKSG9; GSDM; GSDM1 |
| Summary | May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown (Probable).[UniProtKB/Swiss-Prot Function] |
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Resources
Recombinant Protein Resources |
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| LC406011 | GSDMA HEK293T cell transient overexpression lysate (as WB positive control) | 206,00 EUR | |
| LY406011 | Transient overexpression lysate of gasdermin A (GSDMA) | 665,00 EUR | |
| PH319050 | GSDMA MS Standard C13 and N15-labeled recombinant protein (NP_835465) | 3 255,00 EUR |