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> GRP94 Antibody

GRP94 Antibody

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Katalog-Nummer ASM10005-ALP-200

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Marke : Abcepta

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Telefonnummer : +1 850 650 7790

 
  • ICC/IF - GRP94 Antibody ASM10005
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (ASM10005). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (ASM10005) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite.
  • ICC/IF - GRP94 Antibody ASM10005
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (ASM10005). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (ASM10005) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite.
  • WB - GRP94 Antibody ASM10005
    Western Blot analysis of Human HeLa cell lysates showing detection of GRP94 protein using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (ASM10005). Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (ASM10005) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, IP, FCM
Primary Accession P14625
Other Accession NP_003290.1
Host Rat
Isotype IgG2a
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Xenopus, Bovine, Sheep, Horse, Guinea Pig, Dog
Clonality Monoclonal
Description Rat Anti-Chicken GRP94 Monoclonal IgG2a
Target/Specificity Detects ~98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.
Other Names HSP90B1 Antibody, GP96 Antibody, TRA1 Antibody, ECGP Antibody
Clone Names 9G10
Immunogen Purified Grp94 isolated from chicken oviducts
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml of SMC-105 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum| Endoplasmic Reticulum Lumen | Melanosome