Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) - AF488
Katalog-Nummer CSA3211-500ul
Size : 500ul
Marke : Cohesion Biosciences
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Telefonnummer : +1 850 650 7790
- Line colors represent the approximate visible colors of the wavelength maxima.
- Immunofluorescent analysis of beta Tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST. The secondary antibody (green) was Goat Anti-Rabbit IgG (H&L) - AF488 in PBS at room temperature in the dark.
| Product Name | Goat Anti-Rabbit IgG (H&L) - AF488 |
| Cat No: | CSA3211 |
| Source: | Goat |
| Reactivity: | Rb |
| Applications: | E, IH, IF, FC |
| *Application Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
| *Species Reactivity Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
Description: Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) - AF488 Immunogen: Rabbit IgG Purification: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Clonality: Polyclonal Conjugation: AF488 Form: Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide Dilution: IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration) Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.