Goat anti-Kinesin 1 / UKHC
Katalog-Nummer EB05492
Size : 100ug
Marke : Everest Biotech
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Code | Name | Applications | Availability | Size | Tested species | Grade |
|---|---|---|---|---|---|---|
| EB05492 | Goat Anti-Kinesin 1 / UKHC Antibody | Pep-ELISA, WB, IF | 100µg specific antibody in 200µl | Human |
Target Protein
Principal Names: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chainOfficial Symbol: KIF5B
Accession Number(s): NP_004512.1
Human GeneID(s): 3799
Immunogen
Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:8000.Western blot: Approx 110kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110kDa according to NP_004512.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Connell et al. (2019) PMID: 31587092.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Immunofluorescence:This antibody has been successfully used in IF on Human: Connell et al. (2019) PMID: 31587092.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Specific References
This antibody has been successfully used in WB and IF on Human:
James W. Connell, Rachel J. Allison, Catherine E. Rodger, Guy Pearson, Eliska Zlamalova, Evan Reid
ESCRT‑III‑associated proteins and spastin inhibit protrudin‑dependent polarised membrane traffic
Cell Mol Life Sci. 2019 Oct 5. doi: 10.1007/s00018-019-03313-z
PMID: 31587092
This antibody has been successfully used in the following paper:
Ringer K, Riehl J, Müller M, Dewes J, Hoff F, Jacob R
The large GTPase Mx1 binds Kif5B for cargo transport along microtubules.
Traffic. 2018 Dec;19(12):947-964.
PMID: 30246279
This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371
This antibody (previous batch) has been successfully used in WB on Mouse:
Robinson CL, Evans RD, Briggs DA, Ramalho JS, Hume AN.
Deficiency in kinesin-1 recruitment to melanosomes precludes it from facilitating their centrifugal transport.
J Cell Sci. 2017 May 10. pii: jcs.186064.
PMID: 28490438
This antibody (previous batch) has been successfully used in WB:
Diefenbach RJ, Davis A, Miranda-Saksena M, Fernandez MA, Kelly BJ, Jones CA, LaVail JH, Xue J, Lai J, Cunningham AL
The Basic Domain of Herpes Simplex Virus 1 pUS9 Recruits Kinesin- 1 To Facilitate Egress from Neurons
J Virol. 2015 Dec 9;90(4):2102-11
PMID: 26656703
This antibody (previous batch) has been successfully used in the following paper:
Astanina K, Jacob R.
KIF5C, a kinesin motor involved in apical trafficking of MDCK cells.
Cell Mol Life Sci. 2010 Apr;67(8):1331-42.
PMID: 20094756
This antibody (previous batch) has been successfully used in IF on Human:
Gupta V, Palmer KJ, Spence P, Hudson A, Stephens DJ.
Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport.
Traffic. 2008 Nov;9(11):1850-66.
PMID: 18817524
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol