Glycocalicin (GC) Recombinant, Human

Recombinant protein corresponding to aa136-276 with an N-Terminal His-Tag from human GC, expressed inE. coli. Swiss/UniProt Accession: P07359.

LRGLG ELQELYLKGN ELKTLPPGLL TPTPKLEKLS LANNNLTELP AGLLNGLENL DTLLLQENSL YTIPKGFFGS HLLPFAFLHG NPWLCNCEIL YFRRWLQDNA ENVYVWKQGV DVKAMTSNVA SVQCDNSDKF PVYKYP

6.2

17.4kD

17/16kD as determined by SDS-PAGE reducing conditions

Secreted

Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Recombinant, E. coli

≥95%

Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% sucrose. Reconstitute in sterile PBS, pH 7.4 to a concentration of 0.1-1mg/ml. Do not vortex.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.